Figure 3.

Blocking α₂β₁ decreases RhoC GTPase activation. (A) Collagen stimulation increases RhoC activation. LNCaP_col cells were treated in suspension with 1 µg/ml type I collagen for 15 minutes before lysis and were analyzed for RhoC. Data are presented as the fold increase over parental LNCaP control. (B) Blocking α₂β₁ integrin (dark gray bars) decreases active RhoC compared to untreated (white bars) or IgG control (light gray bars). Experiments in A were repeated in the presence of 10 µg/ml neutralizing antibody to α₂β₁ or IgG control and were evaluated for active RhoC protein (n = 4), * P < .0028.