Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Sep;103(3):770–777. doi: 10.1128/jb.103.3.770-777.1970

Multiplicity of the Amino Acid Permeases in Saccharomyces cerevisiae IV. Evidence for a General Amino Acid Permease

M Grenson a, C Hou a,1, M Crabeel a,1
PMCID: PMC248157  PMID: 5474888

Abstract

Kinetic and genetic evidences are presented to show that, in addition to specific amino acid permeases, Saccharomyces cerevisiae has a general amino acid permease which catalyzes the transport of basic and neutral amino acids, but most probably not that of proline. The general amino acid permease appears to be constitutive, and its activity is inhibited when ammonium ions are added to the culture medium. A mutant which has lost the general amino acid permease activity was isolated. Its mutation, named gap (general amino acid permease), is not allelic to the aap (amino acid permease) mutation of Surdin et al., which has a quite different phenotype and cannot be considered as having selectively lost the general amino acid permease activity.

Full text

PDF
770

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crabeel M., Grenson M. Regulation of histidine uptake by specific feedback inhibition of two histidine permeases in Saccharomyces cerevisiae. Eur J Biochem. 1970 May 1;14(1):197–204. doi: 10.1111/j.1432-1033.1970.tb00278.x. [DOI] [PubMed] [Google Scholar]
  2. Gits J. J., Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. doi: 10.1016/0005-2736(67)90040-5. [DOI] [PubMed] [Google Scholar]
  3. Grenson M., Mousset M., Wiame J. M., Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. doi: 10.1016/0304-4165(66)90387-4. [DOI] [PubMed] [Google Scholar]
  4. Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):339–346. doi: 10.1016/0304-4165(66)90388-6. [DOI] [PubMed] [Google Scholar]
  5. Grenson M. The utilization of exogenous pyrimidines and the recycling of uridine-5'-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism. Eur J Biochem. 1969 Dec;11(2):249–260. doi: 10.1111/j.1432-1033.1969.tb00767.x. [DOI] [PubMed] [Google Scholar]
  6. HALVORSON H. O., COHEN G. N. Incorporation des amino-acides endogènes et exogènes dans les protéines de la levure. Ann Inst Pasteur (Paris) 1958 Jul;95(1):73–87. [PubMed] [Google Scholar]
  7. Hawthorne D C, Mortimer R K. Chromosome Mapping in Saccharomyces: Centromere-Linked Genes. Genetics. 1960 Aug;45(8):1085–1110. doi: 10.1093/genetics/45.8.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joiris C. R., Grenson M. Spécificité et régulation d'une perméase des acis aminés dicarboxyliques chez "Saccharomyces crevisiae". Arch Int Physiol Biochim. 1969 Feb;77(1):154–156. [PubMed] [Google Scholar]
  9. MONOD J., PAPPENHEIMER A. M., Jr, COHEN-BAZIRE G. La cinétique de la biosynthèse de la beta-galactosidase chez E. coli considérée comme fonction de la croissance. Biochim Biophys Acta. 1952 Dec;9(6):648–660. doi: 10.1016/0006-3002(52)90227-8. [DOI] [PubMed] [Google Scholar]
  10. Magaña-Schwencke N., Schwencke J. A proline transport system in Saccharomyces chevalieri. Biochim Biophys Acta. 1969 Mar 11;173(2):313–323. doi: 10.1016/0005-2736(69)90114-x. [DOI] [PubMed] [Google Scholar]
  11. Pall M. L. Amino acid transport in Neurospora crassa. I. Properties of two amino acid transport systems. Biochim Biophys Acta. 1969 Jan 28;173(1):113–127. doi: 10.1016/0005-2736(69)90042-x. [DOI] [PubMed] [Google Scholar]
  12. Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
  13. Surdin Y., Sly W., Sire J., Bordes A. M., Robichon-Szulmajster H. Propriétés et contrôle génétique du système d'accumulation des acides aminés chez Saccharomyces cerevisiae. Biochim Biophys Acta. 1965 Oct 18;107(3):546–566. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES