Abstract
A mutant of Escherichia coli K-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designated fep, mapping close to two genes, entA and entD, concerned with enterochelin synthesis. Strain AN102, which carries the fep− allele, accumulates large quantities of enterochelin and gives a growth response to sodium citrate. The cytochrome b1 and total iron content, and the measurement of the uptake of 55Fe3+, indicate an impairment of the enterochelin-dependent iron transport system. The growth response to sodium citrate is related to the presence, in strain AN102, of an inducible citrate-dependent iron transport system.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brot N., Goodwin J., Fales H. In vivo and in vitro formation of 2,3-dihydroxybenzoylserine by Escherichia coli K12. Biochem Biophys Res Commun. 1966 Nov 22;25(4):454–461. doi: 10.1016/0006-291x(66)90227-0. [DOI] [PubMed] [Google Scholar]
- Brot N., Goodwin J. Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. J Biol Chem. 1968 Feb 10;243(3):510–513. [PubMed] [Google Scholar]
- Cox G. B., Gibson F. 2,3-Dihydroxybenzoic acid, a new growth factor for multiple aromatic auxotrophs. J Bacteriol. 1967 Jan;93(1):502–503. doi: 10.1128/jb.93.1.502-503.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cox G. B., Young I. G., McCann L. M., Gibson F. Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol. J Bacteriol. 1969 Aug;99(2):450–458. doi: 10.1128/jb.99.2.450-458.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibson F., Magrath D. I. The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I. Biochim Biophys Acta. 1969 Nov 18;192(2):175–184. doi: 10.1016/0304-4165(69)90353-5. [DOI] [PubMed] [Google Scholar]
- Herbert A. A., Guest J. R. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J Gen Microbiol. 1968 Oct;53(3):363–381. doi: 10.1099/00221287-53-3-363. [DOI] [PubMed] [Google Scholar]
- MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
- O'Brien I. G., Cox G. B., Gibson F. 2,3-dihydroxy-N-benzoylserine: chemical synthesis and comparison with the natural product. Biochim Biophys Acta. 1969 Apr 1;177(2):321–328. doi: 10.1016/0304-4165(69)90142-1. [DOI] [PubMed] [Google Scholar]
- PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
- PITTARD J. EFFECT OF INTEGRATED SEX FACTOR ON TRANSDUCTION OF CHROMOSOMAL GENES IN ESCHERICHIA COLI. J Bacteriol. 1965 Mar;89:680–686. doi: 10.1128/jb.89.3.680-686.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Somerville R. L. Epistasis between deletion mutations in the tryptophan region and mutations affecting the general aromatic pathway in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1798–1799. doi: 10.1128/jb.94.5.1798-1799.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang C. C., Newton A. Iron transport in Escherichia coli: relationship between chromium sensitivity and high iron requirement in mutants of Escherichia coli. J Bacteriol. 1969 Jun;98(3):1135–1141. doi: 10.1128/jb.98.3.1135-1141.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang C. C., Newton A. Iron transport in Escherichia coli: roles of energy-dependent uptake and 2,3-dihydroxybenzoylserine. J Bacteriol. 1969 Jun;98(3):1142–1150. doi: 10.1128/jb.98.3.1142-1150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young I. G., Cox G. B., Gibson F. 2,3-Dihydroxybenzoate as a bacterial growth factor and its route of biosynthesis. Biochim Biophys Acta. 1967 Jul 25;141(2):319–331. doi: 10.1016/0304-4165(67)90106-7. [DOI] [PubMed] [Google Scholar]
- Young I. G., Gibson F. Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli. Biochim Biophys Acta. 1969 May 6;177(3):401–411. doi: 10.1016/0304-4165(69)90302-x. [DOI] [PubMed] [Google Scholar]