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. 1970 Oct;104(1):482–491. doi: 10.1128/jb.104.1.482-491.1970

Segregational Respiratory-Deficient Mutants of a “Petite Negative” Yeast Schizosaccharomyces pombe 972h−

H Heslot 1,2, C Louis 1,2, A Goffeau 1,2,1
PMCID: PMC248233  PMID: 5473904

Abstract

No viable respiratory-deficient mutants of Schizosaccharomyces pombe 972h− could be obtained by acriflavine and ethidium bromide treatments. These mutagens induce 15 to 70% of microcolonies which, after a growth-lag of a few days, further develop into normal, respiratory-competent colonies. These results suggest that unstable petites were induced. Segregational respiratory-deficient mutants resistant to cobalt sulfate inhibition were isolated. Some of these strains are deficient in cytochrome a + a3 and respire at low rates. The morphology of their mitochondrial membranes is modified: either the cristae are absent or they show aberrant concentric or tubular structures. Segregational mutants resistant to the respiratory inhibitors, 2,4-dinitrophenol or decamethylene diguanidine, were obtained. Neither mitochondrial structure nor function seems to be modified in these mutants. A segregational mutant resistant to benzimidazole inhibition does not grow on glycerol, although neither growth on glucose nor respiration appear to be affected.

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Selected References

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  1. AVERS C. J., PFEFFER C. R., RANCOURT M. W. ACRIFLAVINE INDUCTION OF DIFFERENT KINDS OF "PETITE" MITOCHONDRIAL POPULATIONS IN SACCHAROMYCES CEREVISIAE. J Bacteriol. 1965 Aug;90:481–494. doi: 10.1128/jb.90.2.481-494.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avers C. J., Rancourt M. W., Lin F. H. Intracellular mitochondrial diversity in various strains of Saccgarintces cerevisiae. Proc Natl Acad Sci U S A. 1965 Aug;54(2):527–535. doi: 10.1073/pnas.54.2.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BULDER C. J. INDUCTION OF PETITE MUTATION AND INHIBITION OF SYNTHESIS OF RESPIRATORY ENZYMES IN VARIOUS YEASTS. Antonie Van Leeuwenhoek. 1964;30:1–9. doi: 10.1007/BF02046695. [DOI] [PubMed] [Google Scholar]
  4. BULDER C. J. LETHALITY OF THE PETITE MUTATION IN PETITE NEGATIVE YEASTS. Antonie Van Leeuwenhoek. 1964;30:442–454. doi: 10.1007/BF02046758. [DOI] [PubMed] [Google Scholar]
  5. Beck J. C., Mattoon J. R., Hawthorne D. C., Sherman F. Genetic modification of energy-conserving systems in yeast mitochondria. Proc Natl Acad Sci U S A. 1968 May;60(1):186–193. doi: 10.1073/pnas.60.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bernardi G., Carnevali F., Nicolaieff A., Piperno G., Tecce G. Separation and characterization of a satellite DNA from a yeast cytoplasmic "petite" mutant. J Mol Biol. 1968 Nov 14;37(3):493–505. doi: 10.1016/0022-2836(68)90117-4. [DOI] [PubMed] [Google Scholar]
  7. Bernardi G., Faures M., Piperno G., Slonimski P. P. Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast. J Mol Biol. 1970 Feb 28;48(1):23–42. doi: 10.1016/0022-2836(70)90216-0. [DOI] [PubMed] [Google Scholar]
  8. Birkmayer G. D., Bücher T. Ferrochelatase in wild-type and in cytoplasmic mutants of neurospora crassa. FEBS Lett. 1969 Sep;5(1):28–30. doi: 10.1016/0014-5793(69)80284-x. [DOI] [PubMed] [Google Scholar]
  9. Bowers W. D., Jr, McClary D. O., Ogur M. Electron microscopy of a tricarboxylic acid mutant of Saccharomyces. J Bacteriol. 1967 Aug;94(2):482–484. doi: 10.1128/jb.94.2.482-484.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Carnevali F., Morpurgo G., Tecce G. Cytoplasmic DNA from petite colonies of Saccharomyces cerevisiae: a hypothesis on the nature of the mutation. Science. 1969 Mar 21;163(3873):1331–1333. doi: 10.1126/science.163.3873.1331. [DOI] [PubMed] [Google Scholar]
  11. Clark-Walker G. D., Linnane A. W. The biogenesis of mitochondria in Saccharomyces cerevisiae. A comparison between cytoplasmic respiratory-deficient mutant yeast and chlormaphenicol-inhibited wild type cells. J Cell Biol. 1967 Jul;34(1):1–14. doi: 10.1083/jcb.34.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. DE DEKEN R. H. The dissociation of phenotypic and inheritable effects of euflavin in yeast. Exp Cell Res. 1961 Jun;24:145–148. doi: 10.1016/0014-4827(61)90256-7. [DOI] [PubMed] [Google Scholar]
  13. De Deken R. H. The Crabtree effects and its relation to the petite mutation. J Gen Microbiol. 1966 Aug;44(2):157–165. doi: 10.1099/00221287-44-2-157. [DOI] [PubMed] [Google Scholar]
  14. Gordon P. A., Stewart P. R. Ubiquinone formation in wild-type and petite yeast: the effect of catabolite repression. Biochim Biophys Acta. 1969 Apr 1;177(2):358–360. doi: 10.1016/0304-4165(69)90150-0. [DOI] [PubMed] [Google Scholar]
  15. Herman A. I., Griffin P. S. Respiratory-deficient mutants in Saccharomyces lactis. J Bacteriol. 1968 Aug;96(2):457–461. doi: 10.1128/jb.96.2.457-461.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Heslot H., Goffeau A., Louis C. Respiratory metabolism of a "petite negative"yeast Schizosaccharomyces pombe 972h-. J Bacteriol. 1970 Oct;104(1):473–481. doi: 10.1128/jb.104.1.473-481.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Horn P., Wilkie D. Selective advantage of the cytoplasmic respiratory mutant of Saccharomyces cerevisiae in a cobalt medium. Heredity (Edinb) 1966 Nov;21(4):625–635. doi: 10.1038/hdy.1966.62. [DOI] [PubMed] [Google Scholar]
  18. Kovác L., Hrusovská E. Oxidative phosphorylation in yeast. II. An oxidative phosphorylation-deficient mutant. Biochim Biophys Acta. 1968 Jan 15;153(1):43–54. doi: 10.1016/0005-2728(68)90144-8. [DOI] [PubMed] [Google Scholar]
  19. Kovác L., Lachowicz T. M., Slonimski P. P. Biochemical genetics of oxidative phosphorylation. Science. 1967 Dec 22;158(3808):1564–1567. doi: 10.1126/science.158.3808.1564. [DOI] [PubMed] [Google Scholar]
  20. Kovác L., Subík J., Russ G., Kollár K. On the relationship between respiratory activity and lipid composition of the yeast cell. Biochim Biophys Acta. 1967 Aug 8;144(1):94–101. doi: 10.1016/0005-2760(67)90080-x. [DOI] [PubMed] [Google Scholar]
  21. Linnane A. W., Saunders G. W., Gingold E. B., Lukins H. B. The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1968 Mar;59(3):903–910. doi: 10.1073/pnas.59.3.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McClary D. O., Bowers W. D., Jr Mitochondrial changes accompanying acriflavine-induced petite mutation in Saccharomyces fragilis. J Ultrastruct Res. 1968 Oct;25(1):37–45. doi: 10.1016/s0022-5320(68)80058-9. [DOI] [PubMed] [Google Scholar]
  23. Mortimer R. K., Hawthorne D. C. Yeast genetics. Annu Rev Microbiol. 1966;20:151–168. doi: 10.1146/annurev.mi.20.100166.001055. [DOI] [PubMed] [Google Scholar]
  24. Mounolou J. C., Jakob H., Slonimski P. P. Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966 Jul 20;24(2):218–224. doi: 10.1016/0006-291x(66)90723-6. [DOI] [PubMed] [Google Scholar]
  25. NAGAI S., YANAGISHIMA N., NAGAI H. Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol Rev. 1961 Dec;25:404–426. doi: 10.1128/br.25.4.404-426.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. OGUR M., ST. JOHN R., NAGAI S. Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science. 1957 May 10;125(3254):928–929. doi: 10.1126/science.125.3254.928. [DOI] [PubMed] [Google Scholar]
  27. Parker J. H., Mattoon J. R. Mutants of yeast with altered oxidative energy metabolism: selection and genetic characterization. J Bacteriol. 1969 Nov;100(2):647–657. doi: 10.1128/jb.100.2.647-657.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Parker J. H., Trimble I. R., Jr, Mattoon J. R. Oligomycin resistance in normal and mutant yeast. Biochem Biophys Res Commun. 1968 Nov 25;33(4):590–595. doi: 10.1016/0006-291x(68)90336-7. [DOI] [PubMed] [Google Scholar]
  29. REILLY C., SHERMAN R. GLUCOSE REPRESSION OF CYTOCHROME A-SYNTHESIS IN CYTOCHROME-DEFICIENT MUTANTS OF YEAST. Biochim Biophys Acta. 1965 Apr 19;95:640–651. doi: 10.1016/0005-2787(65)90518-6. [DOI] [PubMed] [Google Scholar]
  30. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  31. Schatz G. Impaired binding of mitochondrial adenosine triphosphatase in the cytoplasmic "petite" mutant of Saccharomyces cerevisiae. J Biol Chem. 1968 May 10;243(9):2192–2199. [PubMed] [Google Scholar]
  32. Sels A. A. Origin of the increase in adaptability to oxygen of yeast pretreated with benzimidazole. Biochem Biophys Res Commun. 1969 Mar 31;34(6):740–747. doi: 10.1016/0006-291x(69)90241-1. [DOI] [PubMed] [Google Scholar]
  33. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]
  34. Smith D. G., Marchant R., Maroudas N. G., Wilkie D. A comparative study of the mitochondrial structure of petite strains of Saccharomyces cerevisiae. J Gen Microbiol. 1969 Apr;56(1):47–54. doi: 10.1099/00221287-56-1-47. [DOI] [PubMed] [Google Scholar]
  35. Tewari K. K., Vötsch W., Mahler H. R., Mackler B. Biochemical correlates of respiratory deficiency. VI. Mitochondrial DNA. J Mol Biol. 1966 Oct;20(3):453–481. doi: 10.1016/0022-2836(66)90003-9. [DOI] [PubMed] [Google Scholar]
  36. Thomas D. Y., Wilkie D. Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genet Res. 1968 Feb;11(1):33–41. doi: 10.1017/s0016672300011174. [DOI] [PubMed] [Google Scholar]
  37. Trenina G. A., Bibikova M. V., Sarukhanova L. E. Poluchenie mutantov s defektom okisleniia u razlichnykh vidov drozhzhei. Mikrobiologiia. 1965 Mar-Apr;34(2):300–304. [PubMed] [Google Scholar]
  38. Tuppy H., Swetly P., Wolff I. Structural protein of wild-type (rho+) and respiration-deficient (rho-) yeast mitochondria. A comparative study using electrophoretic and immunological methods. Eur J Biochem. 1968 Aug;5(3):339–345. doi: 10.1111/j.1432-1033.1968.tb00375.x. [DOI] [PubMed] [Google Scholar]
  39. Wilkie D., Lee B. K. A cytoplasmic factor in the resistance of yeast cells to Actidione and its response to UV-irradiation. Genetica. 1965;36(3):267–276. doi: 10.1007/BF01557159. [DOI] [PubMed] [Google Scholar]
  40. Wilkie D., Saunders G., Linnane A. W. Inhibition of respiratory enzyme synthesis in yeast by chloramphenicol: relationship between chloramphenicol tolerance and resistance to other antibacterial antibiotics. Genet Res. 1967 Oct;10(2):199–203. doi: 10.1017/s0016672300010934. [DOI] [PubMed] [Google Scholar]
  41. Wintersberger U., Wintersberger E. Studies on deoxyribonucleic acid polymerases from yeast. 2. Partial purification and characterization of mitochondrial DNA polymerase from wild type and respiration-deficient yeast cells. Eur J Biochem. 1970 Mar 1;13(1):20–27. doi: 10.1111/j.1432-1033.1970.tb00894.x. [DOI] [PubMed] [Google Scholar]
  42. YOTSUYANAGI Y. [Study of yeast mitochondria. II. Mitochondria of respiration-deficient mutants]. J Ultrastruct Res. 1962 Aug;7:141–158. doi: 10.1016/s0022-5320(62)80032-x. [DOI] [PubMed] [Google Scholar]
  43. Yotsuyanagi Y. Un mode de différenciation de la membrane mitochondriale, évoquant le mésosome bactérien. C R Acad Sci Hebd Seances Acad Sci D. 1966 Mar 21;262(12):1348–1351. [PubMed] [Google Scholar]

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