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. 1970 Oct;104(1):499–502. doi: 10.1128/jb.104.1.499-502.1970

Scanning-Beam Electron Microscopy of Mycoplasma pneumoniae

Gary M Kammer 1, J Dennis Pollack 1, Albert S Klainer 1
PMCID: PMC248235  PMID: 5473906

Abstract

The morphology and the existence of a growth cycle of Mycoplasma pneumoniae have not been clearly established. There is disagreement as to whether this organism exists as a spherical or filamentous form, and whether it progresses from filamentous to spherical forms as the organism ages. A scanning-beam electron microscope (SEM) was utilized to provide detailed observations of the cycle of morphological changes during growth phases of M. pneumoniae. Cultures of cells grown and fixed in liquid suspension displayed morphological changes from spherical to filamentous and then to larger round forms. After 8 hr to 2 days of growth (phase I), spherical forms and aggregates were revealed. Two- to 6-day-old growth (phase II) was composed of both straight and branching filaments with bulbous elements situated at intervals along their lengths, and microcolonies composed predominantly of intertwined filaments. Six- to 10-day-old growth (phase III) was characterized by flattened, spherical organisms larger than those observed in phase I, occasional membranes or ghosts, and a paucity of aggregates or microcolonies. Thus, stereo-scan electron microscopic studies suggest that M. pneumoniae undergoes an orderly and sequential metamorphosis during its life cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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