Abstract
Good yields of mutants were obtained by incubating cells of Gaffkya tibisci with N-methyl-N′-nitro-N-nitrosoguanidine in alkaline buffer. Nearly 50% of the survivors were auxotrophic mutants under the conditions described.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ames B. N., Whitfield H. J., Jr Frameshift mutagenesis in Salmonella. Cold Spring Harb Symp Quant Biol. 1966;31:221–225. doi: 10.1101/sqb.1966.031.01.030. [DOI] [PubMed] [Google Scholar]
- Cerdá-Olmedo E., Hanawalt P. C. Diazomethane as the active agent in nitrosoguanidine mutagenesis and lethality. Mol Gen Genet. 1968 May 3;101(3):191–202. doi: 10.1007/BF00271621. [DOI] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delić V., Hopwood D. A., Friend E. J. Mutangenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutat Res. 1970 Feb;9(2):167–182. doi: 10.1016/0027-5107(70)90055-2. [DOI] [PubMed] [Google Scholar]
- Eisenstark A., Eisenstark R., Van Sickle R. Mutation of Salmonella typhimurium by nitrosoguanidine. Mutat Res. 1965 Feb;2(1):1–10. doi: 10.1016/0027-5107(65)90002-3. [DOI] [PubMed] [Google Scholar]
- Megnet R. Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose. Mutat Res. 1965 Aug;2(4):328–331. doi: 10.1016/0027-5107(65)90067-9. [DOI] [PubMed] [Google Scholar]
- Moore D. The mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine on Coprinus lagopus. J Gen Microbiol. 1969 Jan;55(1):121–125. doi: 10.1099/00221287-55-1-121. [DOI] [PubMed] [Google Scholar]