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. 1970 Oct;104(1):590–593. doi: 10.1128/jb.104.1.590-593.1970

Mode of Action of 2-Nitro-1-Butyl Pyridinium Sulfate

Ron Seale a,1, Darrell J Weber a,2
PMCID: PMC248247  PMID: 4990766

Abstract

Ten micrograms of 2-nitro-1-butyl pyridinium sulfate (NBPS) per milliliter inhibited the growth of Bacillus subtilis by blocking thymidylate synthetase. The inhibition could be reversed by the addition of thymine or cytosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTINO J. R., SIMMONS B., DONOHUE D. M. Purification and properties of the formate-activating enzyme from erythrocytes. J Biol Chem. 1962 Apr;237:1314–1318. [PubMed] [Google Scholar]
  2. BLAKLEY R. L. The interconversion of serine and glycine; preparation and properties of catalytic derivatives of pteroylglutamic acid. Biochem J. 1957 Feb;65(2):331–342. doi: 10.1042/bj0650331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S. S., Barner H. D. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1954 Oct;40(10):885–893. doi: 10.1073/pnas.40.10.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cummings D. J., Kusy A. R. Thymineless death in Escherichia coli: deoxyribonucleic acid replication and the immune state. J Bacteriol. 1970 Apr;102(1):106–117. doi: 10.1128/jb.102.1.106-117.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cummings D. J., Mondale L. Thymineless death in Escherichia coli: strain specificity. J Bacteriol. 1967 Jun;93(6):1917–1924. doi: 10.1128/jb.93.6.1917-1924.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GALLANT J., SUSKIND S. R. Ribonucleic acid synthesis and thymineless death. Biochim Biophys Acta. 1962 May 14;55:627–638. doi: 10.1016/0006-3002(62)90841-7. [DOI] [PubMed] [Google Scholar]
  7. LEHMAN I. R., BESSMAN M. J., SIMMS E. S., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J Biol Chem. 1958 Jul;233(1):163–170. [PubMed] [Google Scholar]
  8. Medoff G., Overholt S. Thymineless death in Escherichia coli 15T- and recombinants of 15T- and Escherichia coli K-12. J Bacteriol. 1970 Apr;102(1):213–216. doi: 10.1128/jb.102.1.213-216.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PETERING H. G. Folic acid antagonists. Physiol Rev. 1952 Apr;32(2):197–213. doi: 10.1152/physrev.1952.32.2.197. [DOI] [PubMed] [Google Scholar]
  10. Pauling C., Hanawalt P. Nonconservative DNA replication in bacteria after thymine starvation. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1728–1735. doi: 10.1073/pnas.54.6.1728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reich J., Soska J. The thymineless death and deoxyribosideless death in Lactobacillus acidophilus R-26. Biochem Biophys Res Commun. 1967 Oct 11;29(1):62–67. doi: 10.1016/0006-291x(67)90541-4. [DOI] [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilson M. C., Farmer J. L., Rothman F. Thymidylate synthesis and aminopterin resistance in Bacillus subtilis. J Bacteriol. 1966 Jul;92(1):186–196. doi: 10.1128/jb.92.1.186-196.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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