Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Oct;104(1):601–603. doi: 10.1128/jb.104.1.601-603.1970

Properties of the Amylase from Halobacterium halobium1

Wendy A Good a, Paul A Hartman a
PMCID: PMC248251  PMID: 5473913

Abstract

Halobacterium halobium amylase had optimal activity at pH 6.4 to 6.6 in sodium β-glycerophosphate buffer containing 0.05% NaCl at 55 C; Ca2+ was not required. End products from amylose were maltose, maltotriose, and glucose. The amylase, which was devoid of transglucosylase activity, had a multichain attack mechanism.

Full text

PDF
601

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. French D., Mancusi J. L., Abdullah M., Brammer G. L. Separation of starch oligosaccharides by high temperature paper chromatography. J Chromatogr. 1965 Aug;19(2):445–447. doi: 10.1016/s0021-9673(01)99480-4. [DOI] [PubMed] [Google Scholar]
  2. GIBBONS N. E. The effect of salt concentrations on the biochemical reactions of some halophilic bacteria. Can J Microbiol. 1957 Mar;3(2):249–255. doi: 10.1139/m57-029. [DOI] [PubMed] [Google Scholar]
  3. HOLMES P. K., HALVORSON H. O. PROPERTIES OF A PURIFIED HALOPHILIC MALIC DEHYDROGENASE. J Bacteriol. 1965 Aug;90:316–326. doi: 10.1128/jb.90.2.316-326.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kushner D. J. Halophilic bacteria. Adv Appl Microbiol. 1968;10:73–99. doi: 10.1016/s0065-2164(08)70189-8. [DOI] [PubMed] [Google Scholar]
  5. Lanyi J. K., Stevenson J. Effect of salts and organic solvents on the activity of Halobacterium cutirubrum catalase. J Bacteriol. 1969 May;98(2):611–616. doi: 10.1128/jb.98.2.611-616.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ROBYT J., FRENCH D. Action pattern and specificity of an amylase from Bacillus subtilis. Arch Biochem Biophys. 1963 Mar;100:451–467. doi: 10.1016/0003-9861(63)90112-7. [DOI] [PubMed] [Google Scholar]
  7. Robyt J. F., French D. Multiple attach hypothesis of alpha-amylase action: action of porcine pancreatic, human salivary, and Aspergillus oryzae alpha-amylases. Arch Biochem Biophys. 1967 Oct;122(1):8–16. doi: 10.1016/0003-9861(67)90118-x. [DOI] [PubMed] [Google Scholar]
  8. SEHGAL S. N., GIBBONS N. E. Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol. 1960 Apr;6:165–169. doi: 10.1139/m60-018. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES