Abstract
Glucose was not utilized at significantly different rates in suspensions of multiplying and nonmultiplying adult mouse fibroblasts (L cells). Infection of L cells with Chlamydia psittaci (strain meningopneumonitis) increased the rates of glucose utilization and lactate accumulation during the first 24 hr after infection without changing the rates of glucose utilization by the pentose or tricarboxylic acid cycles. It was concluded that the increased aerobic glycolysis represented a host response to infection and not a parasite activity. The 6BC strain of C. psittaci and the mouse pneumonitis strain of C. trachomatis produced similar changes in the glucose metabolism of L-cells. These results are discussed in relation to the hypothesis that chlamydiae generate to metabolic energy of their own and live by exploiting the energy-rich compounds produced by their hosts.
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- Alexander J. J. Effect of infection with the meningopneumonitis agent on deoxyribonucleic acid and protein synthesis by its L-cell host. J Bacteriol. 1969 Feb;97(2):653–657. doi: 10.1128/jb.97.2.653-657.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alexander J. J. Separation of protein synthesis in meningopneumonitisgent from that in L cells by differential susceptibility to cycloheximide. J Bacteriol. 1968 Feb;95(2):327–332. doi: 10.1128/jb.95.2.327-332.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KATZ J., WOOD H. G. The use of C14O2 yields from glucose-1- and -6-C14 for the evaluation of the pathways of glucose metabolism. J Biol Chem. 1963 Feb;238:517–523. [PubMed] [Google Scholar]
- KITOS P. A., WAYMOUTH C. GLUCOSE METABOLISM BY MOUSE CELLS (NCTC CLONE 929) UNDER CONDITIONS OF DEFINED NUTRITION. Exp Cell Res. 1964 Jun;35:108–118. doi: 10.1016/0014-4827(64)90076-x. [DOI] [PubMed] [Google Scholar]
- Lin H. S. Inhibition of thymidine kinase activity and deoxyribonucleic acid synthesis in L cells infected with the meningopneumonitis agent. J Bacteriol. 1968 Dec;96(6):2054–2065. doi: 10.1128/jb.96.6.2054-2065.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN J. F., MORTON H. J., PARKER R. C. Nutrition of animal cells in tissue culture; initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950 Jan;73(1):1–8. doi: 10.3181/00379727-73-17557. [DOI] [PubMed] [Google Scholar]
- McLIMANS W. F., DAVIS E. V., GLOVER F. L., RAKE G. W. The submerged culture of mammalian cells; the spinner culture. J Immunol. 1957 Nov;79(5):428–433. [PubMed] [Google Scholar]
- Montes de Oca F., Macy M. L., Shannon J. E. Isoenzyme characterization of animal cell cultures. Proc Soc Exp Biol Med. 1969 Nov;132(2):462–469. doi: 10.3181/00379727-132-34238. [DOI] [PubMed] [Google Scholar]
- Tribby I. I. Cell Wall Synthesis by Chlamydia psittaci Growing in L Cells. J Bacteriol. 1970 Dec;104(3):1176–1188. doi: 10.1128/jb.104.3.1176-1188.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E. Adenosine Triphosphate and Other Requirements for the Utilization of Glucose by Agents of the Psittacosis-Trachoma Group. J Bacteriol. 1965 Jul;90(1):243–253. doi: 10.1128/jb.90.1.243-253.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E. Transaminase activity and other enzymatic reactions involving pyruvate and glutamate in Chlamydia (psittacosis-trachoma group). J Bacteriol. 1967 Jan;93(1):177–184. doi: 10.1128/jb.93.1.177-184.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E., Wilson N. N. Role of exogenous adenosine triphosphate in catabolic and synthetic activities of Chlamydia psittaci. J Bacteriol. 1969 Feb;97(2):719–724. doi: 10.1128/jb.97.2.719-724.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]