Abstract
Escherichia coli B/r cells grown on glycerol-containing medium and irradiated with ultraviolet light to about 1% survival respire for about 1 hr and then cease completely for several hours. The results of studies on cell-free respiration and analyses of pyridine nucleotide levels at various times after ultraviolet irradiation show that the cessation of respiration is associated with two changes—loss of glycerol kinase activity and complete disappearance of pyridine nucleotides. Under other cultural conditions in which respiratory inhibition is less complete and more transitory, the losses of pyridine nucleotides are smaller and the rises which follow are correlated with increases in respiratory activity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CARPENTER K. J., KODICEK E. The fluorimetric estimation of N1-methylnicotinamide and its differentiation from coenzyme. Biochem J. 1950 Apr;46(4):421–426. doi: 10.1042/bj0460421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
- Hamkalo B. A., Swenson P. A. Effects of ultraviolet radiation on respiration and growth in radiation-resistant and radiation-sensitive strains of Escherichia coli B. J Bacteriol. 1969 Sep;99(3):815–823. doi: 10.1128/jb.99.3.815-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOBSON K. B., ASTRACHAN L. Specific methods for the microdetermination of pyridine nucleotides and pyridine nucleotide enzymes. Arch Biochem Biophys. 1957 Sep;71(1):69–80. doi: 10.1016/0003-9861(57)90008-5. [DOI] [PubMed] [Google Scholar]
- KOCH J. P., HAYASHI S., LIN E. C. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3106–3108. [PubMed] [Google Scholar]
- LIN E. C., KOCH J. P., CHUSED T. M., JORGENSEN S. E. Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2145–2150. doi: 10.1073/pnas.48.12.2145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swenson P. A., Schenley R. L. Evidence for the control of respiration by DNA in ultraviolet-irradiated Escherichia coli B-r cells. Mutat Res. 1970 May;9(5):443–453. doi: 10.1016/0027-5107(70)90028-x. [DOI] [PubMed] [Google Scholar]