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. 1970 Dec;104(3):1280–1285. doi: 10.1128/jb.104.3.1280-1285.1970

Periodic Density Fluctuation During the Yeast Cell Cycle and the Selection of Synchronous Cultures

Leland H Hartwell 1
PMCID: PMC248288  PMID: 16559104

Abstract

Yeast cells undergo periodic fluctuations in density during the cell division cycle such that a minimum in density occurs at the time of cell separation whereas a maximum occurs between the time of deoxyribonucleic acid replication and nuclear division. Synchronous cultures can be selected from asynchronously growing cell cultures by withdrawing the cells of least or greatest density after banding in Renografin-sucrose density gradients. This technique is rapid, reproducible, and almost unlimited in capacity.

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Selected References

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