Skip to main content
PMC home page
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1973;48(5):559–562.

Immunogenicity of purified, inactivated chikungunya virus in monkeys*

Eiichi Nakao, Susumu Hotta
PMCID: PMC2482923  PMID: 4204490

Abstract

Chikungunya (CHIK) virus, harvested from infected BHK-21 cell cultures and highly purified by a method combining zinc acetate precipitation, Sephadex—Sepharose column chromatography, and sucrose density gradient centrifugation, was subjected to ultraviolet (UV) irradiation and treatment with formalin. Inactivation of the virus by UV light was apparently a first-order reaction. The virus treated with 0.05% formalin at 4°C was inactivated completely after 7 weeks, but when treated with 0.025% formalin retained its plaque infectivity at least 17 weeks. Both UV- and formalin-inactivated viruses induced production of anti-CHIK neutralizing antibodies in Japanese monkeys with no preimmune antibodies. Judging by the titres of antibodies produced, the immunogenic effect of the UV-irradiated virus was superior to that of the formalin-treated virus.

Full text

PDF
559

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eckels K. H., Harrison V. R., Hetrick F. M. Chikungunya virus vaccine prepared by Tween-ether extraction. Appl Microbiol. 1970 Feb;19(2):321–325. doi: 10.1128/am.19.2.321-325.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gruber J. Immunogenicity of purified venezuelan equine encephalitis virus inactivated by ionizing radiation. Infect Immun. 1971 Apr;3(4):574–579. doi: 10.1128/iai.3.4.574-579.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HOTCHIN J. E. Use of methyl cellulose gel as a substitute for agar in tissue-culture overlays. Nature. 1955 Feb 19;175(4451):352–352. doi: 10.1038/175352a0. [DOI] [PubMed] [Google Scholar]
  5. Harrison V. R., Binn L. N., Randall R. Comparative immunogenicities of chikungunya vaccines prepared in avian and mammalian tissues. Am J Trop Med Hyg. 1967 Nov;16(6):786–791. [PubMed] [Google Scholar]
  6. Harrison V. R., Eckels K. H., Bartelloni P. J., Hampton C. Production and evaluation of a formalin-killed Chikungunya vaccine. J Immunol. 1971 Sep;107(3):643–647. [PubMed] [Google Scholar]
  7. Igarashi A., Nithiuthai P., Rojanasuphot S. Immunological properties of Chikungunya virus and its components. Biken J. 1970 Sep;13(3):229–231. [PubMed] [Google Scholar]
  8. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  9. Nakao E. Biolgoical and immunological studies on chikungunya virus: a comparative observation of two strains of African and Asian origins. Kobe J Med Sci. 1972 Jun;18(2):133–141. [PubMed] [Google Scholar]
  10. SCHULZE I. T., SCHLESINGER R. W. Plaque assay of dengue and other group B arthropod-borne viruses under methyl cellulose overlay media. Virology. 1963 Jan;19:40–48. doi: 10.1016/0042-6822(63)90022-9. [DOI] [PubMed] [Google Scholar]
  11. Yoshinaka Y., Hotta S. Purification of arboviruses grown in tissue culture. Proc Soc Exp Biol Med. 1971 Jul;137(3):1047–1053. doi: 10.3181/00379727-137-35725. [DOI] [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES