Skip to main content
NCBI home page
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1973;48(5):535–541.

A rapid reproducible test for determining rabies neutralizing antibody*

Jean S Smith, Pamela A Yager, George M Baer
PMCID: PMC2482941  PMID: 4544144

Abstract

Rabies neutralizing antibody levels in human and animal sera were tested by a rapid fluorescent focus inhibition technique, in which BHK-21 cells were infected with tissue-culture-adapted rabiesvirus. The results, obtained in 24 hours, were comparable with those of the standard mouse neutralization test.

Full text

PDF
535

Images in this article

537Fig. 1
on p.537
537Fig. 2
on p.537
537Fig. 3
on p.537

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATANASIU P., LEPINE P. Multiplication du virus rabique des rues sur la tumeur épendymaire de la souris en culture de tissus; effet cytolytique. Ann Inst Pasteur (Paris) 1959 Jan;96(1):72–78. [PubMed] [Google Scholar]
  2. Abelseth M. K. Propagation of Rabies Virus in Pig Kidney Cell Culture. Can Vet J. 1964 Apr;5(4):84–87. [PMC free article] [PubMed] [Google Scholar]
  3. Atanasiu P. Quantitative assay and potency test of antirabies serum. Monogr Ser World Health Organ. 1966;23:167–172. [PubMed] [Google Scholar]
  4. DEAN D. J., EVANS W. M., THOMPSON W. R. STUDIES ON THE LOW EGG PASSAGE FLURY STRAIN OF MODIFIED LIVE RABIES VIRUS PRODUCED IN EMBRYONATING CHICKEN EGGS AND TISSUE CULTURE. Am J Vet Res. 1964 May;25:756–763. [PubMed] [Google Scholar]
  5. Dean D. J. Laboratory techniques in rabies. The fluorescent antibody test. Monogr Ser World Health Organ. 1966;23:59–68. [PubMed] [Google Scholar]
  6. Debbie J. G., Andrulonis J. A., Abelseth M. K. Rabies antibody determination by immunofluorescence in tissue culture. Infect Immun. 1972 Jun;5(6):902–904. doi: 10.1128/iai.5.6.902-904.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ENDERS J. F., PEEBLES T. C. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc Soc Exp Biol Med. 1954 Jun;86(2):277–286. doi: 10.3181/00379727-86-21073. [DOI] [PubMed] [Google Scholar]
  8. FERNANDES M. V., WIKTOR T. J., KOPROWSKI H. MECHANISM OF THE CYTOPATHIC EFFECT OF RABIES VIRUS IN TISSUE CULTURE. Virology. 1963 Sep;21:128–131. doi: 10.1016/0042-6822(63)90312-x. [DOI] [PubMed] [Google Scholar]
  9. GOLDWASSER R. A., KISSLING R. E. Fluorescent antibody staining of street and fixed rabies virus antigens. Proc Soc Exp Biol Med. 1958 Jun;98(2):219–223. doi: 10.3181/00379727-98-23996. [DOI] [PubMed] [Google Scholar]
  10. Gough P. M., Dierks R. E. Passive haemagglutination test for antibodies against rabies virus. Bull World Health Organ. 1971;45(6):741–745. [PMC free article] [PubMed] [Google Scholar]
  11. Halonen P. E., Murphy F. A., Fields B. N., Reese D. R. Hemagglutinin of rabies and some other bullet-shaped viruses. Proc Soc Exp Biol Med. 1968 Apr;127(4):1037–1042. doi: 10.3181/00379727-127-32864. [DOI] [PubMed] [Google Scholar]
  12. Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KAPLAN M. M. Demonstration of rabies virus in tissue culture with fluorescent antibody technique. Bull World Health Organ. 1960;22:434–435. [PMC free article] [PubMed] [Google Scholar]
  14. KING D. A., CROGHAN D. L., SHAW E. L. A RAPID QUANTITATIVE IN VITRO SERUM NEUTRALIZATION TEST FOR RABIES ANTIBODY. Can Vet J. 1965 Aug;6:187–193. [PMC free article] [PubMed] [Google Scholar]
  15. KISSLING R. E. Growth of rabies virus in non-nervous tissue culture. Proc Soc Exp Biol Med. 1958 Jun;98(2):223–225. doi: 10.3181/00379727-98-23997. [DOI] [PubMed] [Google Scholar]
  16. KISSLING R. E., REESE D. R. ANTI-RABIES VACCINE OF TISSUE CULTURE ORIGIN. J Immunol. 1963 Sep;91:362–368. [PubMed] [Google Scholar]
  17. Kaplan M. M., Wiktor T. J., Maes R. F., Campbell J. B., Koprowski H. Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle growth curve. J Virol. 1967 Feb;1(1):145–151. doi: 10.1128/jvi.1.1.145-151.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
  19. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  20. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  21. SALK J. E., YOUNGNER J. S., WARD E. N. Use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue-culture system. Am J Hyg. 1954 Sep;60(2):214–230. doi: 10.1093/oxfordjournals.aje.a119714. [DOI] [PubMed] [Google Scholar]
  22. Sedwick W. D., Wiktor T. J. Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. J Virol. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wiktor T. J., Kuwert E., Koprowski H. Immune lysis of rabies virus-infected cells. J Immunol. 1968 Dec;101(6):1271–1282. [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES