Abstract
The need for a technique that is more sensitive than the use of Romanowsky-stained thick blood films for detecting malaria parasites at low concentration in the blood is well recognized. One of the more promising methods appeared to be fluorochrome staining with acridine orange. However, reports on the efficacy of the technique were contradictory and it was not clear to what extent blood films taken under survey conditions would contain fluorescing artefacts that might confuse diagnosis. An investigation indicated that, provided reasonable care was taken, blood films made under survey conditions contained few confusing artefacts. However, it was found that, while acridine orange staining might have a slight advantage when large malaria parasites were present, it was inferior to routine Romanowsky staining for the detection of young trophozoites, the inferiority becoming more pronounced as the parasite concentration decreased.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ambroise-Thomas P., Michel-Brun J., Despeignes J. Identification rapide des parasites sanguicoles par coloration à l'acridine orange et microscopie de fluorescence. Bull Soc Pathol Exot Filiales. 1965 Jul-Aug;58(4):639–643. [PubMed] [Google Scholar]
- FUHRMANN G. [Use of fluorescence microscopic methods for the demonstration of malarial parasites]. Z Tropenmed Parasitol. 1962 Apr;13:24–29. [PubMed] [Google Scholar]
- Janis B., Hunter D. T., Richards D. F. A new method for detecting Plasmodia. Mil Med. 1971 Jan;136(1):23–26. [PubMed] [Google Scholar]
- Seitz H. M. Vergleichende Untersuchung vo n Akridinorgange- und Wright-Fäbung zur Malariadiagnose. Z Tropenmed Parasitol. 1971 Jun;22(2):136–138. [PubMed] [Google Scholar]
- Sodeman T. M. The use of fluorochromes for the detection of malaria parasites. Am J Trop Med Hyg. 1970 Jan;19(1):40–42. doi: 10.4269/ajtmh.1970.19.40. [DOI] [PubMed] [Google Scholar]