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. 1971 Jan;105(1):101–106. doi: 10.1128/jb.105.1.101-106.1971

Small Ribosomal Ribonucleic Acid Species of Saccharomyces cerevisiae

Stephen A Udem 1, Karen Kaufman 2, Jonathan R Warner 3
PMCID: PMC248327  PMID: 5540996

Abstract

Yeast ribosomes contain two small molecular-weight species of ribonucleic acid (RNA), in addition to transiently associated transfer RNA. The 5S RNA species is part of the large ribosomal subunit and appears to be exactly the same size as 5S RNA from other organisms. There is another RNA molecule, approximately 5.8S or 150 nucleotides in size, which is noncovalently attached to the 25S ribosomal RNA and can be freed by gentle heating or urea treatment. Neither 5 nor 5.8S RNA are methylated. The 5.8S RNA is probably derived from a part of the 35S precursor RNA, whereas the 5S RNA is made de novo. These results substantiate the notion that ribosome biosynthesis in yeast is analogous to that of the higher eukaryotes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubert M., Scott J. F., Reynier M., Monier R. Rearrangement of the conformation of Escherichia coli 5S RNA. Proc Natl Acad Sci U S A. 1968 Sep;61(1):292–299. doi: 10.1073/pnas.61.1.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):661–680. doi: 10.1016/0022-2836(68)90188-5. [DOI] [PubMed] [Google Scholar]
  3. Brownlee G. G., Sanger F. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):337–353. doi: 10.1016/s0022-2836(67)80109-8. [DOI] [PubMed] [Google Scholar]
  4. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edelman M., Verma I. M., Littauer U. Z. Mitochondrial ribosomal RNA from Aspergillus nidulans: characterization of a novel molecular species. J Mol Biol. 1970 Apr 14;49(1):67–83. doi: 10.1016/0022-2836(70)90376-1. [DOI] [PubMed] [Google Scholar]
  6. Forget B. G., Weissman S. M. The nucleotide sequence of ribosomal 5 S ribonucleic acid from KB cells. J Biol Chem. 1969 Jun 25;244(12):3148–3165. [PubMed] [Google Scholar]
  7. Hutchison H. T., Hartwell L. H. Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967 Nov;94(5):1697–1705. doi: 10.1128/jb.94.5.1697-1705.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knight E., Jr, Darnell J. E. Distribution of 5 s RNA in HeLa cells. J Mol Biol. 1967 Sep 28;28(3):491–502. doi: 10.1016/s0022-2836(67)80099-8. [DOI] [PubMed] [Google Scholar]
  9. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin T. E., Hartwell L. H. Resistance of active yeast ribosomes to dissociation by KCl. J Biol Chem. 1970 Mar 25;245(6):1504–1506. [PubMed] [Google Scholar]
  12. Martin T. E., Wool I. G. Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats. Proc Natl Acad Sci U S A. 1968 Jun;60(2):569–574. doi: 10.1073/pnas.60.2.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
  14. ROSSET R., MONIER R., JULIEN J. LES RIBOSOMES D'ESCHERICHIA COLI. I. MISE EN 'EVIDENCE D'UN RNA RIBOSOMIQUE DE FAIBLE POIDS MOL'ECULAIRE. Bull Soc Chim Biol (Paris) 1964;46:87–109. [PubMed] [Google Scholar]
  15. Retèl J., van den Bos R. C., Planta R. J. Characteristics of the methylation in vivo of ribosomal RNA in yeast. Biochim Biophys Acta. 1969 Dec 16;195(2):370–380. doi: 10.1016/0005-2787(69)90643-1. [DOI] [PubMed] [Google Scholar]
  16. Schlessinger D., Apirion D. Escherichia coli ribosomes: recent developments. Annu Rev Microbiol. 1969;23:387–426. doi: 10.1146/annurev.mi.23.100169.002131. [DOI] [PubMed] [Google Scholar]
  17. Sillevis Smitt W. W., Nanni G., Rozijn T. H., Tonino G. J. Sedimentation characteristics of RNA from isolated yeast nuclei. Exp Cell Res. 1970 Mar;59(3):440–446. doi: 10.1016/0014-4827(70)90651-8. [DOI] [PubMed] [Google Scholar]
  18. Sy J., McCarty K. S. Characterization of 5.8-S RNA from a complex with 26-S ribosomal RNA from Arbacia punctulata. Biochim Biophys Acta. 1970 Jan 21;199(1):86–94. doi: 10.1016/0005-2787(70)90697-0. [DOI] [PubMed] [Google Scholar]
  19. Taber R. L., Jr, Vincent W. S. The synthesis and processing of ribosomal RNA precursor molecules in yeast. Biochim Biophys Acta. 1969 Aug 20;186(2):317–325. doi: 10.1016/0005-2787(69)90009-4. [DOI] [PubMed] [Google Scholar]
  20. WARNER J. R., RICH A. THE NUMBER OF SOLUBLE RNA MOLECULES ON RETICULOCYTE POLYRIBOSOMES. Proc Natl Acad Sci U S A. 1964 Jun;51:1134–1141. doi: 10.1073/pnas.51.6.1134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Warner J. R., Soeiro R. Nascent ribosomes from HeLa cells. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1984–1990. doi: 10.1073/pnas.58.5.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weinberg R. A., Penman S. Small molecular weight monodisperse nuclear RNA. J Mol Biol. 1968 Dec;38(3):289–304. doi: 10.1016/0022-2836(68)90387-2. [DOI] [PubMed] [Google Scholar]

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