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. 1971 Jan;105(1):185–189. doi: 10.1128/jb.105.1.185-189.1971

Physiological Studies of Encystment in Azotobacter vinelandii1

H L Sadoff a, E Berke a,2, B Loperfido a
PMCID: PMC248340  PMID: 5541004

Abstract

Azotobacter vinelandii, in late exponential growth phase, encysts when the glucose in the medium is replaced with β-hydroxybutyrate. A final cell division then occurs without apparent deoxyribonucleic acid (DNA) synthesis, resulting in a reduction from two to one nucleoids per cell and a final DNA content of 3.2 × 10−14 g per cell. This is also the DNA content per cyst. A β-hydroxybutyrate dehydrogenase is derepressed by the addition of the inducer and is identical to the enzyme in acetate-grown cells in its pH optimum, Michaelis constant for substrate, temperature-activity response, and mobility during electrophoresis in acrylamide gel.

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Selected References

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