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. 1971 Jan;105(1):241–248. doi: 10.1128/jb.105.1.241-248.1971

Catabolite Repression in the d-Serine Deaminase System of Escherichia coli K-12

Elizabeth McFall 1, Frederic R Bloom 1
PMCID: PMC248347  PMID: 4322347

Abstract

The induced synthesis of d-serine deaminase in Escherichia coli is subject to three catabolic effects: inhibition on inducer uptake, transient repression, and catabolite repression. Inhibition on d-serine uptake is not significant at the d-serine concentration normally used for induction. Transient repression and catabolite repression of d-serine deaminase synthesis are abolished by mutations in dsdCy, which appears to be an operator locus. The decline in the rate of constitutive synthesis observed in dsdCx mutants growing with glycerol as carbon source at temperatures above 37 C is due to catabolite repression. The low level of constitutivity at 37 C and the partial cis dominance of dsdCx mutants are not artifacts of catabolite repression. It is suggested that a product of one of the genes of the dsd operon may regulate the expression of the operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cosloy S. D., McFall E. L-Serine-sensitive mutants of Escherichia coli K-12. J Bacteriol. 1970 Sep;103(3):840–841. doi: 10.1128/jb.103.3.840-841.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Englesberg E., Sheppard D., Squires C., Meronk F., Jr An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic). J Mol Biol. 1969 Jul 28;43(2):281–298. doi: 10.1016/0022-2836(69)90268-x. [DOI] [PubMed] [Google Scholar]
  3. GORINI L., KAUFMAN H. Selecting bacterial mutants by the penicillin method. Science. 1960 Feb 26;131(3400):604–605. doi: 10.1126/science.131.3400.604. [DOI] [PubMed] [Google Scholar]
  4. ISHIBASHI M., SUGINO Y., HIROTA Y. CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM. J Bacteriol. 1964 Mar;87:554–561. doi: 10.1128/jb.87.3.554-561.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Llanes B., McFall E. Effect of galactose on beta-galactosidase synthesis in Escherichia coli K-12. J Bacteriol. 1969 Jan;97(1):217–222. doi: 10.1128/jb.97.1.217-222.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loomis W. F., Jr, Magasanik B. Glucose-lactose diauxie in Escherichia coli. J Bacteriol. 1967 Apr;93(4):1397–1401. doi: 10.1128/jb.93.4.1397-1401.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MAGASANIK B. Catabolite repression. Cold Spring Harb Symp Quant Biol. 1961;26:249–256. doi: 10.1101/sqb.1961.026.01.031. [DOI] [PubMed] [Google Scholar]
  8. MAKMAN R. S., SUTHERLAND E. W. ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1965 Mar;240:1309–1314. [PubMed] [Google Scholar]
  9. MCFALL E. GENETIC STRUCTURE OF THE D-SERINE DEAMINASE SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Sep;9:746–753. doi: 10.1016/s0022-2836(64)80179-0. [DOI] [PubMed] [Google Scholar]
  10. MCFALL E. PLEIOTROPIC MUTATIONS IN THE D-SERINE DEAMINASE SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Sep;9:754–762. doi: 10.1016/s0022-2836(64)80180-7. [DOI] [PubMed] [Google Scholar]
  11. McFall E. "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1967 Dec;94(6):1989–1993. doi: 10.1128/jb.94.6.1989-1993.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McFall E. Dominance studies with stable merodiploids in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1967 Dec;94(6):1982–1988. doi: 10.1128/jb.94.6.1982-1988.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFall E., Heincz M. C. Thermosensitive regulation of D-serine deaminase synthesis in a mutant of Escherichia coli K 12. Mol Gen Genet. 1970;106(4):371–377. doi: 10.1007/BF00324054. [DOI] [PubMed] [Google Scholar]
  14. McFall E. Mapping of the d-serine deaminase region in Escherichia coli K-12. Genetics. 1967 Jan;55(1):91–99. doi: 10.1093/genetics/55.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Monard D., Janecek J., Rickenberg H. V. The enzymic degradation of 3',5' cyclic AMP in strains of E. Coli sensitive and resistant to catobolite repression. Biochem Biophys Res Commun. 1969 May 22;35(4):584–591. doi: 10.1016/0006-291x(69)90388-x. [DOI] [PubMed] [Google Scholar]
  16. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perlman R. L., De Crombrugghe B., Pastan I. Cyclic AMP regulates catabolite and transient repression in E. coli. Nature. 1969 Aug 23;223(5208):810–812. doi: 10.1038/223810a0. [DOI] [PubMed] [Google Scholar]
  18. Perlman R. L., Pastan I. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli. Biochem Biophys Res Commun. 1969 Sep 24;37(1):151–157. doi: 10.1016/0006-291x(69)90893-6. [DOI] [PubMed] [Google Scholar]
  19. Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
  20. Silverstone A. E., Magasanik B., Reznikoff W. S., Miller J. H., Beckwith J. R. Catabolite sensitive site of the lac operon. Nature. 1969 Mar 15;221(5185):1012–1014. doi: 10.1038/2211012b0. [DOI] [PubMed] [Google Scholar]
  21. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tyler B., Magasanik B. Molecular basis of transient repression of beta-galactosidase in Escherichia coli. J Bacteriol. 1969 Feb;97(2):550–556. doi: 10.1128/jb.97.2.550-556.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WILLSON C., PERRIN D., COHN M., JACOB F., MONOD J. NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Apr;8:582–592. doi: 10.1016/s0022-2836(64)80013-9. [DOI] [PubMed] [Google Scholar]

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