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. 1971 Jan;105(1):369–375. doi: 10.1128/jb.105.1.369-375.1971

Purification and Thermal Stability of Intact Bacillus subtilis Flagella

K Dimmitt 1, M Simon 1
PMCID: PMC248364  PMID: 4993323

Abstract

Flagella were prepared and purified in a relatively intact form from bacterial lysates. Immunochemical tests showed that over 95% of the protein in the final preparation consisted of flagellar antigen. These flagella are more stable to thermal denaturation than flagella filaments obtained by shearing. Their thermal properties more closely resemble those of flagella in the native state on bacteria. The presence of the hook structure is responsible for this extra stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAM D., KOFFLER H. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN. J Mol Biol. 1964 Jul;9:168–185. doi: 10.1016/s0022-2836(64)80098-x. [DOI] [PubMed] [Google Scholar]
  2. ADA G. L., NOSSAL G. J., PYE J., ABBOT A. ANTIGENS IN IMMUNITY. I. PREPARATION AND PROPERTIES OF FLAGELLAR ANTIGENS FROM SALMONELLA ADELAIDE. Aust J Exp Biol Med Sci. 1964 Jun;42:267–282. [PubMed] [Google Scholar]
  3. Abram D., Koffler H., Vatter A. E. Basal structure and attachment of flagella in cells of Proteus vulgaris. J Bacteriol. 1965 Nov;90(5):1337–1354. doi: 10.1128/jb.90.5.1337-1354.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Abram D., Vatter A. E., Koffler H. Attachment and structural features of flagella of certain bacilli. J Bacteriol. 1966 May;91(5):2045–2068. doi: 10.1128/jb.91.5.2045-2068.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Asakura S., Eguchi G., Iino T. Unidirectional growth of Salmonella flagella in vitro. J Mol Biol. 1968 Jul 14;35(1):227–236. doi: 10.1016/s0022-2836(68)80050-6. [DOI] [PubMed] [Google Scholar]
  6. Cohen-Bazire G., London J. Basal organelles of bacterial flagella. J Bacteriol. 1967 Aug;94(2):458–465. doi: 10.1128/jb.94.2.458-465.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DePamphilis M. L., Adler J. Purification of intact flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):376–383. doi: 10.1128/jb.105.1.376-383.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grant G. F., Simon M. I. Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis. J Bacteriol. 1969 Jul;99(1):116–124. doi: 10.1128/jb.99.1.116-124.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grant G. F., Simon M. Use of radioactive antibodies for characterizing antigens and application to the study of flagella synthesis. J Bacteriol. 1968 Jan;95(1):81–86. doi: 10.1128/jb.95.1.81-86.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoeniger J. F., Van Iterson W., Van Zanten E. N. Basal bodies of bacterial flagella in Proteus mirabilis. II. Electron microscopy of negatively stained material. J Cell Biol. 1966 Dec;31(3):603–618. doi: 10.1083/jcb.31.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KOBAYASHI T., RINKER J. N., KOFFLER H. Purification and and chemical properties of flagellin. Arch Biochem Biophys. 1959 Oct;84:342–362. doi: 10.1016/0003-9861(59)90598-3. [DOI] [PubMed] [Google Scholar]
  12. Klein D., Foster J. F., Koffler H. Changes in polarimetric parameters associated with the polymerization of flagellin into flagellar filaments. Biochem Biophys Res Commun. 1969 Aug 22;36(5):844–850. doi: 10.1016/0006-291x(69)90686-x. [DOI] [PubMed] [Google Scholar]
  13. Koffler H., Mallett G. E., Adye J. MOLECULAR BASIS OF BIOLOGICAL STABILITY TO HIGH TEMPERATURES. Proc Natl Acad Sci U S A. 1957 Jun 15;43(6):464–477. doi: 10.1073/pnas.43.6.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROBERTS N. R., LEINER K. Y., WU M. L., FARR A. L. The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954 Mar;207(1):1–17. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MARTINEZ R. J., ROSENBERG E. THERMAL TRANSITION OF SPIRILLUM SERPENS FLAGELLA. J Mol Biol. 1964 May;8:702–707. doi: 10.1016/s0022-2836(64)80119-4. [DOI] [PubMed] [Google Scholar]
  17. Martinez R. J., Brown D. M., Glazer A. N. The formation of bacterial flagella. 3. Characterization of the subunits of the flagella of Bacillus subtilis and Spirillum serpens. J Mol Biol. 1967 Aug 28;28(1):45–51. doi: 10.1016/s0022-2836(67)80076-7. [DOI] [PubMed] [Google Scholar]
  18. Vaituzis Z., Doetsch R. N. Relationship between cell wall, cytoplasmic membrane, and bacterial motility. J Bacteriol. 1969 Oct;100(1):512–521. doi: 10.1128/jb.100.1.512-521.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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