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. 1971 Jan;105(1):384–395. doi: 10.1128/jb.105.1.384-395.1971

Fine Structure and Isolation of the Hook-Basal Body Complex of Flagella from Escherichia coli and Bacillus subtilis

M L DePamphilis 1, Julius Adler 2
PMCID: PMC248366  PMID: 4993325

Abstract

The hook-basal body complex comprising the basal end of purified intact flagella from Escherichia coli and Bacillus subtilis was studied in detail with an electron microscope. The E. coli hook can be described as having five or six concentric helical coils. The basal body from E. coli is 27 nm in length and consists of four rings, 22.5 nm in diameter, arranged in two pairs and mounted on a rod. The top pair of rings is connected near their periphery, resembling a closed cylinder. In B. subtilis the basal body looks like that from E. coli, except that the top pair of rings is missing. Hook-basal body complexes from both organisms could be isolated by dissociating the filaments with either urea or acid. Based on our results, two types of basal body structures are proposed, as exemplified by E. coli and B. subtilis, which directly reflect the structure of the gram-negative and gram-positive cell envelopes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAM D., KOFFLER H. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN. J Mol Biol. 1964 Jul;9:168–185. doi: 10.1016/s0022-2836(64)80098-x. [DOI] [PubMed] [Google Scholar]
  2. Abram D., Koffler H., Vatter A. E. Basal structure and attachment of flagella in cells of Proteus vulgaris. J Bacteriol. 1965 Nov;90(5):1337–1354. doi: 10.1128/jb.90.5.1337-1354.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Abram D., Mitchen J. R., Koffler H., Vatter A. E. Differentiation within the bacterial flagellum and isolation of the proximal hook. J Bacteriol. 1970 Jan;101(1):250–261. doi: 10.1128/jb.101.1.250-261.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Abram D., Vatter A. E., Koffler H. Attachment and structural features of flagella of certain bacilli. J Bacteriol. 1966 May;91(5):2045–2068. doi: 10.1128/jb.91.5.2045-2068.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Armstrong J. B., Adler J. Complementation of nonchemotactic mutants of Escherichia coli. Genetics. 1969 Jan;61(1):61–66. doi: 10.1093/genetics/61.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Armstrong J. B., Adler J. Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics. 1967 Jul;56(3):363–373. doi: 10.1093/genetics/56.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COHEN-BAZIRE G., KUNISAWA R. The fine structure of Rhodospirillum rubrum. J Cell Biol. 1963 Feb;16:401–419. doi: 10.1083/jcb.16.2.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cohen-Bazire G., London J. Basal organelles of bacterial flagella. J Bacteriol. 1967 Aug;94(2):458–465. doi: 10.1128/jb.94.2.458-465.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. De Petris S. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. J Ultrastruct Res. 1967 Jul;19(1):45–83. doi: 10.1016/s0022-5320(67)80059-5. [DOI] [PubMed] [Google Scholar]
  10. DePamphilis M. L., Adler J. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane. J Bacteriol. 1971 Jan;105(1):396–407. doi: 10.1128/jb.105.1.396-407.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DePamphilis M. L., Adler J. Purification of intact flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):376–383. doi: 10.1128/jb.105.1.376-383.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ensign J. C., Wolfe R. S. Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J Bacteriol. 1966 Feb;91(2):524–534. doi: 10.1128/jb.91.2.524-534.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Finch J. T., Leberman R., Yu-Shang C., Klug A. Rotational symmetry of the two turn disk aggregate of tobacco mosaic virus protein. Nature. 1966 Oct 22;212(5060):349–350. doi: 10.1038/212349a0. [DOI] [PubMed] [Google Scholar]
  14. GLAUERT A. M., KERRIDGE D., HORNE R. W. THE FINE STRUCTURE AND MODE OF ATTACHMENT OF THE SHEATHED FLAGELLUM OF VIBRIO METCHNIKOVII. J Cell Biol. 1963 Aug;18:327–336. doi: 10.1083/jcb.18.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HORNE R. W., WILDY P. VIRUS STRUCTURE REVEALED BY NEGATIVE STAINING. Adv Virus Res. 1963;10:101–170. doi: 10.1016/s0065-3527(08)60698-3. [DOI] [PubMed] [Google Scholar]
  16. Hoeniger J. F., Van Iterson W., Van Zanten E. N. Basal bodies of bacterial flagella in Proteus mirabilis. II. Electron microscopy of negatively stained material. J Cell Biol. 1966 Dec;31(3):603–618. doi: 10.1083/jcb.31.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Iino T. Genetics and chemistry of bacterial flagella. Bacteriol Rev. 1969 Dec;33(4):454–475. doi: 10.1128/br.33.4.454-475.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Joys T. M., Stocker B. A. Complementation of non-flagellate Salmonella mutants. J Gen Microbiol. 1965 Oct;41(1):47–55. doi: 10.1099/00221287-41-1-47. [DOI] [PubMed] [Google Scholar]
  19. Keeler R. F., Ritchie A. E., Bryner J. H., Elmore J. The preparation and characterization of cell walls and the preparation of flagella of Vibrio fetus. J Gen Microbiol. 1966 Jun;43(3):439–454. doi: 10.1099/00221287-43-3-439. [DOI] [PubMed] [Google Scholar]
  20. Klug A., De Rosier D. J. Optical filtering of electron micrographs: reconstruction of one-sided images. Nature. 1966 Oct 1;212(5057):29–32. doi: 10.1038/212029a0. [DOI] [PubMed] [Google Scholar]
  21. LOWY J., HANSON J. ELECTRON MICROSCOPE STUDIES OF BACTERIAL FLAGELLA. J Mol Biol. 1965 Feb;11:293–313. doi: 10.1016/s0022-2836(65)80059-6. [DOI] [PubMed] [Google Scholar]
  22. Lowy J. Structure of the proximal ends of bacterial flagella. J Mol Biol. 1965 Nov;14(1):297–299. doi: 10.1016/s0022-2836(65)80251-0. [DOI] [PubMed] [Google Scholar]
  23. Nauman R. K., Holt S. C., Cox C. D. Purification, ultrastructure, and composition of axial filaments from Leptospira. J Bacteriol. 1969 Apr;98(1):264–280. doi: 10.1128/jb.98.1.264-280.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. PEASE P. Some observations upon the development and mode of attachment of the flagella in Vibrio and Spirillum species. Exp Cell Res. 1956 Feb;10(1):234–237. doi: 10.1016/0014-4827(56)90092-1. [DOI] [PubMed] [Google Scholar]
  25. Remsen C. C., Watson S. W., Waterbury J. B., Trüper H. G. Fine structure of Ectothiorhodospira mobilis Pelsh. J Bacteriol. 1968 Jun;95(6):2374–2392. doi: 10.1128/jb.95.6.2374-2392.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ritchie A. E., Keeler R. F., Bryner J. H. Anatomical features of Vibrio fetus: Electron microscopic survey. J Gen Microbiol. 1966 Jun;43(3):427–438. doi: 10.1099/00221287-43-3-427. [DOI] [PubMed] [Google Scholar]
  27. Tawara J. The root of flagella of Vibrio cholerae. Jpn J Microbiol. 1965 Mar;9(1):49–54. doi: 10.1111/j.1348-0421.1965.tb00274.x. [DOI] [PubMed] [Google Scholar]
  28. Vaituzis Z., Doetsch R. N. Relationship between cell wall, cytoplasmic membrane, and bacterial motility. J Bacteriol. 1969 Oct;100(1):512–521. doi: 10.1128/jb.100.1.512-521.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Van Iterson W., Hoeniger J. F., Van Zanten E. N. Basal bodies of bacterial flagella in Proteus mirabilis. I. Electron microscopy of sectioned material. J Cell Biol. 1966 Dec;31(3):585–601. doi: 10.1083/jcb.31.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van Iterson W. Symposium on the fine structure and replication of bacteria and their parts. II. Bacterial cytoplasm. Bacteriol Rev. 1965 Sep;29(3):299–325. doi: 10.1128/br.29.3.299-325.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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