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. 1971 Feb;105(2):467–471. doi: 10.1128/jb.105.2.467-471.1971

Mutant of Escherichia coli K-12 Defective in d-Glucosamine Biosynthesis

Matti Sarvas a,1
PMCID: PMC248396  PMID: 5541524

Abstract

A mutant was isolated from a derivative of Escherichia coli K-12 after mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. This mutant contained normal levels of 2-amino-2-deoxy-d-glucose-6-phosphate ketol-isomerase (deaminating) (EC 5.3.1.10), but no detectable activity of l-glutamine:d-fructose-6-phosphate amino-transferase (EC 2.6.1.16). It required either N-acetyl-d-glucosamine or d-glucosamine for growth, and went into rapid lysis when the supply of these compounds was exhausted. In medium containing 11% sucrose, the cells were converted into spheroplasts in the absence of d-glucosamine.

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Selected References

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