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. 1971 Feb;105(2):477–482. doi: 10.1128/jb.105.2.477-482.1971

Mutation Affecting Activity of Several Distinct Amino Acid Transport Systems in Saccharomyces cerevisiae

M Grenson a, C Hennaut a,1
PMCID: PMC248400  PMID: 5541525

Abstract

Mutations at the apf locus selectively depress the activity of a number of distinct amino acid permeases in Saccharomyces cerevisiae. The activity of the general amino acid permease and specific amino acid permeases is decreased, but the uptake of pyrimidines and adenine is unaffected. Mutations at the apf locus are allelic to the aap mutation isolated by Surdin et al. Amino acid uptake is normal in a heterozygous diploid (apf/+) and in a tetraploid strain with only one functional allele at the apf locus.

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Selected References

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  1. Barnes E. M., Jr, Kaback H. R. Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1190–1198. doi: 10.1073/pnas.66.4.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beaud G. Mode d'action de la glycine sul l'inhibition de la croissance d'Agrobacterium tumefaciens. I. Effets sur le métabolisme des RNA messagers des bactéries, souche B6. Biochim Biophys Acta. 1966 Dec 21;129(3):563–575. [PubMed] [Google Scholar]
  3. Crabeel M., Grenson M. Regulation of histidine uptake by specific feedback inhibition of two histidine permeases in Saccharomyces cerevisiae. Eur J Biochem. 1970 May 1;14(1):197–204. doi: 10.1111/j.1432-1033.1970.tb00278.x. [DOI] [PubMed] [Google Scholar]
  4. EGAN J. B., MORSE M. L. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT. Biochim Biophys Acta. 1965 Feb 15;97:310–319. doi: 10.1016/0304-4165(65)90096-6. [DOI] [PubMed] [Google Scholar]
  5. Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. 3. Studies of the transport process. Biochim Biophys Acta. 1966 Jan 4;112(1):63–73. doi: 10.1016/s0926-6585(96)90009-6. [DOI] [PubMed] [Google Scholar]
  6. Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. II. Characterization of the defect of a pleiotropic transport mutant. Biochim Biophys Acta. 1965 Sep 27;109(1):172–183. doi: 10.1016/0926-6585(65)90101-9. [DOI] [PubMed] [Google Scholar]
  7. Gits J. J., Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. doi: 10.1016/0005-2736(67)90040-5. [DOI] [PubMed] [Google Scholar]
  8. Grenson M., Hou C., Crabeel M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J Bacteriol. 1970 Sep;103(3):770–777. doi: 10.1128/jb.103.3.770-777.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grenson M., Mousset M., Wiame J. M., Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. doi: 10.1016/0304-4165(66)90387-4. [DOI] [PubMed] [Google Scholar]
  10. Grenson M. The utilization of exogenous pyrimidines and the recycling of uridine-5'-phosphate derivatives in Saccharomyces cerevisiae, as studied by means of mutants affected in pyrimidine uptake and metabolism. Eur J Biochem. 1969 Dec;11(2):249–260. doi: 10.1111/j.1432-1033.1969.tb00767.x. [DOI] [PubMed] [Google Scholar]
  11. HALVORSON H. O., COHEN G. N. Incorporation des amino-acides endogènes et exogènes dans les protéines de la levure. Ann Inst Pasteur (Paris) 1958 Jul;95(1):73–87. [PubMed] [Google Scholar]
  12. Hengstenberg W., Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. V. The accumulation of phosphorylated carbohydrate derivatives, and evidence for a new enzyme-splitting lactose phosphate. Proc Natl Acad Sci U S A. 1967 Jul;58(1):274–279. doi: 10.1073/pnas.58.1.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hengstenberg W., Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. VI. The nature of the derivatives accumulated. J Biol Chem. 1968 Apr 25;243(8):1881–1885. [PubMed] [Google Scholar]
  14. Hengstenberg W., Penberthy W. K., Hill K. L., Morse M. L. Metabolism of lactose by Staphylococcus aureus. J Bacteriol. 1968 Dec;96(6):2187–2188. doi: 10.1128/jb.96.6.2187-2188.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hengstenberg W., Penberthy W. K., Hill K. L., Morse M. L. Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides. J Bacteriol. 1969 Aug;99(2):383–388. doi: 10.1128/jb.99.2.383-388.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hennaut C., Hilger F., Grenson M. Space limitation for permease insertion in the cytoplasmic membrane of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1970 May 22;39(4):666–671. doi: 10.1016/0006-291x(70)90257-3. [DOI] [PubMed] [Google Scholar]
  17. Jacobson E. S., Metzenberg R. L. A new gene which affects uptake of neutral and acidic amino acids in Neurospora crassa. Biochim Biophys Acta. 1968 Feb 1;156(1):140–147. doi: 10.1016/0304-4165(68)90113-x. [DOI] [PubMed] [Google Scholar]
  18. Joiris C. R., Grenson M. Spécificité et régulation d'une perméase des acis aminés dicarboxyliques chez "Saccharomyces crevisiae". Arch Int Physiol Biochim. 1969 Feb;77(1):154–156. [PubMed] [Google Scholar]
  19. KEPES A. [Kinetic studies on galactoside permease of Escherichia coli]. Biochim Biophys Acta. 1960 May 6;40:70–84. doi: 10.1016/0006-3002(60)91316-0. [DOI] [PubMed] [Google Scholar]
  20. KOCH A. L. THE ROLE OF PERMEASE IN TRANSPORT. Biochim Biophys Acta. 1964 Jan 27;79:177–200. doi: 10.1016/0926-6577(64)90050-6. [DOI] [PubMed] [Google Scholar]
  21. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kaback H. R., Milner L. S. Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1008–1015. doi: 10.1073/pnas.66.3.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kennedy E. P., Scarborough G. A. Mechanism of hydrolysis of O-nitrophenyl-beta-galactoside in Staphylococcus aureus and its significance for theories of sugar transport. Proc Natl Acad Sci U S A. 1967 Jul;58(1):225–228. doi: 10.1073/pnas.58.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LEDERBERG J., LEDERBERG E. M., ZINDER N. D., LIVELY E. R. Recombination analysis of bacterial heredity. Cold Spring Harb Symp Quant Biol. 1951;16:413–443. doi: 10.1101/sqb.1951.016.01.030. [DOI] [PubMed] [Google Scholar]
  25. Laue P., MacDonald R. E. Identification of thiomethyl-beta-D-galactoside 6-phosphate accumulated by Staphylococcus aureus. J Biol Chem. 1968 Feb 10;243(3):680–682. [PubMed] [Google Scholar]
  26. Magaña-Schwencke N., Schwencke J. A proline transport system in Saccharomyces chevalieri. Biochim Biophys Acta. 1969 Mar 11;173(2):313–323. doi: 10.1016/0005-2736(69)90114-x. [DOI] [PubMed] [Google Scholar]
  27. SORSOLI W. A., SPENCE K. D., PARKS L. W. AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE. J Bacteriol. 1964 Jul;88:20–24. doi: 10.1128/jb.88.1.20-24.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
  29. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Surdin Y., Sly W., Sire J., Bordes A. M., Robichon-Szulmajster H. Propriétés et contrôle génétique du système d'accumulation des acides aminés chez Saccharomyces cerevisiae. Biochim Biophys Acta. 1965 Oct 18;107(3):546–566. [PubMed] [Google Scholar]
  31. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  32. Tanaka S., Lin E. C. Two classes of pleiotropic mutants of Aerobacter aerogenes lacking components of a phosphoenolpyruvate-dependent phosphotransferase system. Proc Natl Acad Sci U S A. 1967 Apr;57(4):913–919. doi: 10.1073/pnas.57.4.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wang R. J., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants. J Mol Biol. 1968 Feb 28;32(1):59–66. doi: 10.1016/0022-2836(68)90145-9. [DOI] [PubMed] [Google Scholar]
  34. Winkler H. H., Wilson T. H. Inhibition of beta-galactoside transport by substrates of the glucose transport system in Escherichia coli. Biochim Biophys Acta. 1967;135(5):1030–1051. doi: 10.1016/0005-2736(67)90073-9. [DOI] [PubMed] [Google Scholar]

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