Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Feb;105(2):573–579. doi: 10.1128/jb.105.2.573-579.1971

Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

M Murgier 1, J P Belaich 1
PMCID: PMC248425  PMID: 5541534

Abstract

A microcalorimetric method using a Tian-Calvet apparatus was utilized for measuring the Km and Vmax of intact yeast cells for some carbohydrate substrates. These values were obtained for fructose, galactose, and maltose. The heats of ethanolic fermentations were also directly determined, and the results were very close to those obtained by using theoretical calculations and standard values.

Full text

PDF
573

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES G. F. UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM. Arch Biochem Biophys. 1964 Jan;104:1–18. doi: 10.1016/s0003-9861(64)80028-x. [DOI] [PubMed] [Google Scholar]
  2. BELAICH J. P., PRAT H. [Glucose-limiting thermogenesis and growth of Pseudomonas lindneri]. C R Seances Soc Biol Fil. 1963 Jun 10;157:316–322. [PubMed] [Google Scholar]
  3. BELAUICH J. P., SENEZ J. C. INFLUENCE OF AERATION AND OF PANTOTHENATE ON GROWTH YIELDS OF ZYMOMONAS MOBILIS. J Bacteriol. 1965 May;89:1195–1200. doi: 10.1128/jb.89.5.1195-1200.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLAKLEY E. R., BOYER P. D. The effect of 6-deoxy-6-fluoro-d-glucose on yeast fermentation and on hexokinase. Biochim Biophys Acta. 1955 Apr;16(4):576–582. doi: 10.1016/0006-3002(55)90281-x. [DOI] [PubMed] [Google Scholar]
  5. BURGER M., HEJMOVA L., KLEINZELLER A. Transport of some mono- and di-saccharides into yeast cells. Biochem J. 1959 Feb;71(2):233–242. doi: 10.1042/bj0710233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Belaich J. P., Sari J. C. Microcalorimetric studies on the formation of magnesium complexes of adenine nucleotides. Proc Natl Acad Sci U S A. 1969 Oct;64(2):763–770. doi: 10.1073/pnas.64.2.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Belaich J. P., Senez J. C., Murgier M. Microcalorimetric study of glucose permeation in microbial cells. J Bacteriol. 1968 May;95(5):1750–1757. doi: 10.1128/jb.95.5.1750-1757.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FORREST W. W., WALKER D. J., HOPGOOD M. F. Enthalpy changes associated with the lactic fermentation of glucose. J Bacteriol. 1961 Nov;82:685–690. doi: 10.1128/jb.82.5.685-690.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SCHARFF T. G., KREMER E. H., 3rd A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast. Arch Biochem Biophys. 1962 Apr;97:192–198. doi: 10.1016/0003-9861(62)90064-4. [DOI] [PubMed] [Google Scholar]
  10. SOLS A. Selective fermentation and phosphorylation of sugars by sauternes yeast. Biochim Biophys Acta. 1956 Apr;20(1):62–68. doi: 10.1016/0006-3002(56)90263-3. [DOI] [PubMed] [Google Scholar]
  11. Wilkins P. O., Cirillo V. P. Sorbose counterflow as a measure of intracellular glucose in baker's yeast. J Bacteriol. 1965 Dec;90(6):1605–1610. doi: 10.1128/jb.90.6.1605-1610.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. van Steveninck J., Dawson E. C. Active and passive galactose transport in yeast. Biochim Biophys Acta. 1968 Jan 3;150(1):47–55. doi: 10.1016/0005-2736(68)90007-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES