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. 1989 Apr;63(4):1818–1821. doi: 10.1128/jvi.63.4.1818-1821.1989

Selectable recombinant herpesvirus saimiri is capable of persisting in a human T-cell line.

R Grassmann 1, B Fleckenstein 1
PMCID: PMC248459  PMID: 2538660

Abstract

Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cells contained episomal viral DNA in high copy number. This selectable vector should be generally applicable for gene expression in human T cells.

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Selected References

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