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. 1971 Mar;105(3):831–836. doi: 10.1128/jb.105.3.831-836.1971

Isolation and Properties of a New Species of Ribonucleic Acid Synthesized in Sporulating Cells of Saccharomyces cerevisiae

Kiyoshi Kadowaki 1, H O Halvorson 1
PMCID: PMC248507  PMID: 5547991

Abstract

A new species of ribonucleic acid (RNA) was detected in sporulating culture of Saccharomyces cerevisiae. This RNA was isolated by sucrose density gradient centrifugation and polyacrylamide gel electrophoresis and partially characterized. It has a sedimentation coefficient of approximately 20S and a nucleotide composition distinct from other known RNA species in yeast, and it hybridizes with nuclear but not with mitochondrial deoxyribonucleic acid.

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Selected References

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  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. Acetate utilization and macromolecular synthesis during sporulation of yeast. J Bacteriol. 1969 Oct;100(1):180–186. doi: 10.1128/jb.100.1.180-186.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. Conditional mutants of meiosis in yeast. J Bacteriol. 1970 Oct;104(1):202–210. doi: 10.1128/jb.104.1.202-210.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Esposito M. S., Esposito R. E. The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants. Genetics. 1969 Jan;61(1):79–89. doi: 10.1093/genetics/61.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kadowaki K., Halvorson H. O. Appearance of a new species of ribonucleic acid during sporulation in Saccharomyces cerevisiae. J Bacteriol. 1971 Mar;105(3):826–830. doi: 10.1128/jb.105.3.826-830.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  8. Popa L. M., Repanovici R. The study of toluidine blue-ribosomal RNA complexes. Biochim Biophys Acta. 1969 May 20;182(1):158–168. doi: 10.1016/0005-2787(69)90530-9. [DOI] [PubMed] [Google Scholar]
  9. Retèl J., Planta R. J. Ribosomal precursor RNA in Saccharomyces carlsbergensis. Eur J Biochem. 1967 Dec;3(2):248–258. doi: 10.1111/j.1432-1033.1967.tb19524.x. [DOI] [PubMed] [Google Scholar]
  10. Rogers M. E., Loening U. E., Fraser R. S. Ribosomal RNA precursors in plants. J Mol Biol. 1970 May 14;49(3):681–692. doi: 10.1016/0022-2836(70)90291-3. [DOI] [PubMed] [Google Scholar]
  11. Roth R., Halvorson H. O. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. doi: 10.1128/jb.98.2.831-832.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  13. Taber R. L., Jr, Vincent W. S. Effects of cycloheximide on ribosomal RNA synthesis in yeast. Biochem Biophys Res Commun. 1969 Feb 21;34(4):488–494. doi: 10.1016/0006-291x(69)90408-2. [DOI] [PubMed] [Google Scholar]
  14. Taber R. L., Jr, Vincent W. S. The synthesis and processing of ribosomal RNA precursor molecules in yeast. Biochim Biophys Acta. 1969 Aug 20;186(2):317–325. doi: 10.1016/0005-2787(69)90009-4. [DOI] [PubMed] [Google Scholar]
  15. Tewari K. K., Jayaraman J., Mahler H. R. Separation and characterization of mitochondrial DNA from yeast. Biochem Biophys Res Commun. 1965 Oct 26;21(2):141–148. doi: 10.1016/0006-291x(65)90100-2. [DOI] [PubMed] [Google Scholar]
  16. de Kloet S. R. Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis. Biochem J. 1966 Jun;99(3):566–581. doi: 10.1042/bj0990566. [DOI] [PMC free article] [PubMed] [Google Scholar]

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