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. 1971 Mar;105(3):902–907. doi: 10.1128/jb.105.3.902-907.1971

Aurintricarboxylic Acid, a Preferential Inhibitor of Initiation of Protein Synthesis

Frederic Siegelman 1, David Apirion 1
PMCID: PMC248516  PMID: 5547994

Abstract

The effect of aurintricarboxylic acid (ATA) was tested on various aspects of protein synthesis directed by the natural messenger ribonucleic acid (RNA) isolated from R17 RNA bacteriophage. The effects of various levels of ATA (up to 1,000 μm) were tested on overall protein synthesis as well as on binding of messenger RNA and fmet-transfer RNA to ribosomes and on the addition of the 50S ribosome to the 30S ribosome initiation complex. All of the reactions tested could be inhibited by ATA, and none of the tested steps was found to be uniquely sensitive to it. However, the total initiation steps were more sensitive to this chemical than the elongation steps; thus, under appropriate conditions this chemical can preferentially inhibit initiation while elongation of the polypeptide chain is not appreciably affected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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