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. 1971 Mar;105(3):1063–1072. doi: 10.1128/jb.105.3.1063-1072.1971

Biosynthesis of T1 Antigen in Salmonella: Origin of d-Galactofuranose and d-Ribofuranose Residues

Matti Sarvas a,1, Hiroshi Nikaido a
PMCID: PMC248537  PMID: 4926677

Abstract

The “T1 side chain” portion of cell wall lipopolysaccharide from T1 strains of Salmonella contains d-galactofuranose and d-ribofuranose residues. Isotope labeling studies, using intact cells of mutants each blocked at either of the two different steps of d-galactose metabolism (uridine diphosphate-glucose 4-epimerase and galactose-1-P uridylyl transferase) or at phosphoglucoisomerase, led to the following conclusions. (i) d-Galactofuranose residues are synthesized from d-galactopyranose or its derivatives, rather than by a direct conversion from other hexopyranoses or their derivatives. (ii) The pyranose-to-furanose conversion does not appear to take place at the level of the free d-galactose or d-galactose 1-phosphate. This result suggests that the conversion may occur at the stage of uridine diphosphate-d-galactose. (iii) In a mutant lacking phosphoglucoisomerase, d-ribofuranose residues in T1 side chains contained 14C derived from exogenous d-fructose-U-14C, but little 3H from exogenous d-glucose-1-3H. Thus, no evidence was found for a direct pathway of aldohexose-to-ribose conversion involving a loss of one of the carbons in the C2-C6 moiety of aldohexoses. This suggests, but does not prove, that the T1 ribofuranose residues are synthesized by conventional mechanisms involving hexose monophosphate shunt and transketolase-transaldolase reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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