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. 1971 Mar;105(3):1184–1199. doi: 10.1128/jb.105.3.1184-1199.1971

Dissociation and Reassembly of Escherichia coli Outer Membrane and of Lipopolysaccharide, and Their Reassembly onto Flagellar Basal Bodies

M L DePamphilis 1
PMCID: PMC248550  PMID: 4100836

Abstract

Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg2+. Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg2+. Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg2+ (divalent cation)-mediated and hydrophobic bonds.

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These references are in PubMed. This may not be the complete list of references from this article.

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