Abstract
Spherical bodies, that are obtained by adding penicillin and lysozyme to Clostridium botulinum types E and A cultures which are growing in an osmotically stabilized medium, are shown to be protoplasts by electron microscopy. The L-forms of these two culture types have morphologically different inclusion bodies.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown G. W., King G., Sugiyama H. Penicillin-Lysozyme Conversion of Clostridium botulinum Types A and E into Protoplasts and Their Stabilization as L-Form Cultures. J Bacteriol. 1970 Dec;104(3):1325–1331. doi: 10.1128/jb.104.3.1325-1331.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dienes L., Bullivant S. Morphology and reproductive processes of the L forms of bacteria. II. Comparative study of L forms and Mycoplasma with the electron microscope. J Bacteriol. 1968 Feb;95(2):672–687. doi: 10.1128/jb.95.2.672-687.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatten B. A., Schulze M. L., Huang S. Y., Sulkin S. E. Ultrastructure of Brucella abortus L-forms induced by penicillin in a liquid and in a semisolid medium. J Bacteriol. 1969 Aug;99(2):611–618. doi: 10.1128/jb.99.2.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strasdine G. A. Amylopectin accumulation in Clostridium botulinum type E. Can J Microbiol. 1968 Oct;14(10):1059–1062. doi: 10.1139/m68-178. [DOI] [PubMed] [Google Scholar]
- WILLIAMSON D. H., WILKINSON J. F. The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species. J Gen Microbiol. 1958 Aug;19(1):198–209. doi: 10.1099/00221287-19-1-198. [DOI] [PubMed] [Google Scholar]
- Weibull C., Bickel W. D., Haskins W. T., Milner K. C., Ribi E. Chemical, biological, and structural properties of stable Proteus L forms and their parent bacteria. J Bacteriol. 1967 Mar;93(3):1143–1159. doi: 10.1128/jb.93.3.1143-1159.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]