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. 1971 Jun;106(3):732–738. doi: 10.1128/jb.106.3.732-738.1971

Mutant of Rhodopseudomonas spheroides Unable to Grow Aerobically1

T Wittenberg a,2, W R Sistrom a
PMCID: PMC248686  PMID: 5557591

Abstract

We isolated a mutant of Rhodopseudomonas spheroides that grows normally under photosynthetic conditions but is unable to grow exponentially under aerobic conditions. Photosynthetically grown cultures of the mutant increase in mass and cell number for about 10 hr after transfer to aerobic conditions. During this time, no heme pigments are synthesized and the Q(O2) declines. In the mutant, synthesis of heme pigments is obligatorily coupled to synthesis of bacteriochlorophyll.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLAYTON R. K. Competition between light and dark metabolism in Rhodospirillum rubrum. Arch Mikrobiol. 1955;22(2):195–203. doi: 10.1007/BF00409305. [DOI] [PubMed] [Google Scholar]
  2. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  3. Cusanovich M. A., Kamen M. D. Light-induced electron transport in Chromatium strain D. I. Isolation and characterization of Chromatium chromatophores. Biochim Biophys Acta. 1968 Feb 12;153(2):376–396. doi: 10.1016/0005-2728(68)90082-0. [DOI] [PubMed] [Google Scholar]
  4. GRIFFITHS M., STANIER R. Y. Some mutational changes in the photosynthetic pigment system of Rhodopseudomonas spheroides. J Gen Microbiol. 1956 Jul;14(3):698–715. doi: 10.1099/00221287-14-3-698. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
  7. SISTROM W. R. EFFECT OF OXYGEN ON GROWTH AND THE SYNTHESIS OF BACTERIOCHLOROPHYLL IN RHODOSPIRILLUM MOLISCHIANUM. J Bacteriol. 1965 Feb;89:403–408. doi: 10.1128/jb.89.2.403-408.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SISTROM W. R. Observations on the relationship between the formation of photopigments and the synthesis of protein in Rhodopseudomonas spheroides. J Gen Microbiol. 1962 Sep;28:599–605. doi: 10.1099/00221287-28-4-599. [DOI] [PubMed] [Google Scholar]
  9. Sistrom W. R. The spectrum of bacteriochlorophyll in vivo: observations on mutants of Rhodopseudomonas spheroides unable to grow photosynthetically. Photochem Photobiol. 1966 Nov-Dec;5(11):845–856. doi: 10.1111/j.1751-1097.1966.tb05931.x. [DOI] [PubMed] [Google Scholar]
  10. Thore A., Keister D. L., San Pietro A. Studies on the respiratory system of aerobically (dark) and anaerobically (light) grown Rhodospirillum rubrum. Arch Mikrobiol. 1969;67(4):378–396. doi: 10.1007/BF00412584. [DOI] [PubMed] [Google Scholar]

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