Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jun;106(3):784–790. doi: 10.1128/jb.106.3.784-790.1971

Phenylalanine Biosynthesis in Escherichia coli K-12: Mutants Derepressed for Chorismate Mutase P-Prephenate Dehydratase

S W K Im 1, J Pittard 1
PMCID: PMC248692  PMID: 4934063

Abstract

Mutants were isolated which are derepressed for the synthesis of chorismate mutase P-prephenate dehydratase. No other enzymes involved in the synthesis of phenylalanine are derepressed in these strains. These mutants are able to grow in concentrations of o- and p-fluorophenylalanine that inhibit the growth of AB3259, the strain from which they were derived. They also excrete phenylalanine. Genetic analysis shows that the mutations causing this derepression are closely linked to the structural gene for this enzyme (cotransduction frequency of 95% or more with pheA). The gene in which they occur has been designated pheO since this gene has all of the properties predicted for an operator gene controlling the pheA structural gene. Finally, the pheO mutant alleles have been shown to be dominant in diploids.

Full text

PDF
784

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown K. D. Regulation of aromatic amino acid biosynthesis Escherichia coli K12. Genetics. 1968 Sep;60(1):31–48. doi: 10.1093/genetics/60.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COTTON R. G., GIBSON F. THE BIOSYNTHESIS OF PHENYLALANINE AND TYROSINE; ENZYMES CONVERTING CHORISMIC ACID INTO PREPHENIC ACID AND THEIR RELATIONSHIPS TO PREPHENATE DEHYDRATASE AND PREPHENATE DEHYDROGENASE. Biochim Biophys Acta. 1965 Apr 12;100:76–88. doi: 10.1016/0304-4165(65)90429-0. [DOI] [PubMed] [Google Scholar]
  4. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  5. Gibson F., Pittard J. Pathways of biosynthesis of aromatic amino acids and vitamins and their control in microorganisms. Bacteriol Rev. 1968 Dec;32(4 Pt 2):465–492. [PMC free article] [PubMed] [Google Scholar]
  6. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Pittard J., Camakaris J., Wallace B. J. Inhibition of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (trp) in Escherichia coli. J Bacteriol. 1969 Mar;97(3):1242–1247. doi: 10.1128/jb.97.3.1242-1247.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pittard J., Wallace B. J. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1494–1508. doi: 10.1128/jb.91.4.1494-1508.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SILBERT D. F., JORGENSEN S. E., LIN E. C. Repression of transaminase A by tyrosine in Escherichia coli. Biochim Biophys Acta. 1963 Jun 11;73:232–240. doi: 10.1016/0006-3002(63)90307-x. [DOI] [PubMed] [Google Scholar]
  11. Wallace B. J., Pittard J. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli. J Bacteriol. 1967 Jan;93(1):237–244. doi: 10.1128/jb.93.1.237-244.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wallace B. J., Pittard J. Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli. J Bacteriol. 1969 Mar;97(3):1234–1241. doi: 10.1128/jb.97.3.1234-1241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES