Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jun;106(3):819–823. doi: 10.1128/jb.106.3.819-823.1971

Effect of Glutamate on Exogenous Citrate Catabolism of Neisseria meningitidis and of Other Species of Neisseria1

James C Hill a
PMCID: PMC248698  PMID: 4997540

Abstract

Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO2 produced from citrate is proportional to the amount of glutamate added. When the cells are disrupted, citrate is degraded at a rapid rate and the stimulatory effect of glutamate is completely eliminated. Pronounced stimulation of citrate metabolism by glutamate was demonstrated in 12 of 13 strains of N. meningitidis tested and only 1 of 6 strains of N. lactamicus. The remaining strains of N. lactamicus and one each of N. gonorrhoeae, N. flavescens, and N. flava did not utilize significant amounts of citrate in the absence or presence of glutamate. N. catarrhalis shared with Mima polymorpha and Moraxella glucidolytica a capability to catabolize citrate at a rapid rate without added glutamate. It is concluded that tests of glutamate-stimulated citrate metabolism may contribute to species characterization in the genus Neisseria.

Full text

PDF
819

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann P., Doudoroff M., Stanier R. Y. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968 May;95(5):1520–1541. doi: 10.1128/jb.95.5.1520-1541.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumann P., Doudoroff M., Stanier R. Y. Study of the Moraxella group. I. Genus Moraxella and the Neisseria catarrhalis group. J Bacteriol. 1968 Jan;95(1):58–73. doi: 10.1128/jb.95.1.58-73.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CATLIN B. W., CUNNINGHAM L. S. Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. J Gen Microbiol. 1961 Oct;26:303–312. doi: 10.1099/00221287-26-2-303. [DOI] [PubMed] [Google Scholar]
  4. Hollis D. G., Wiggins G. L., Weaver R. E. Neisseria lactamicus sp. n., a lactose-fermenting species resembling Neisseria meningitidis. Appl Microbiol. 1969 Jan;17(1):71–77. doi: 10.1128/am.17.1.71-77.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hollis D. G., Wiggins G. L., Weaver R. E., Schubert J. H. Current status of lactose-fermenting Neisseria. Ann N Y Acad Sci. 1970 Oct 30;174(2):444–449. doi: 10.1111/j.1749-6632.1970.tb45572.x. [DOI] [PubMed] [Google Scholar]
  6. JYSSUM K. Intermediate reactions of the tricarboxylic acid cycle in meningococci. Acta Pathol Microbiol Scand. 1960;48:121–132. doi: 10.1111/j.1699-0463.1960.tb04748.x. [DOI] [PubMed] [Google Scholar]
  7. Kingsbury D. T. Deoxyribonucleic acid homologies among species of the genus Neisseria. J Bacteriol. 1967 Oct;94(4):870–874. doi: 10.1128/jb.94.4.870-874.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kingsbury D. T., Fanning G. R., Johnson K. E., Brenner D. J. Thermal stability of interspecies Neisseria DNA duplexes. J Gen Microbiol. 1969 Feb;55(2):201–208. doi: 10.1099/00221287-55-2-201. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Mallavia L. P., Weiss E. Catabolic activities of Neisseria meningitidis: utilization of glutamate. J Bacteriol. 1970 Jan;101(1):127–132. doi: 10.1128/jb.101.1.127-132.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SLATERUS K. W. Serological typing of meningococci by means of micro-precipitation. Antonie Van Leeuwenhoek. 1961;27:305–315. doi: 10.1007/BF02538460. [DOI] [PubMed] [Google Scholar]
  12. Weiss E. Catabolic Activities of Neisseria meningitidis: Utilization of Succinate. J Bacteriol. 1970 Jan;101(1):133–137. doi: 10.1128/jb.101.1.133-137.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weiss E. Transaminase activity and other enzymatic reactions involving pyruvate and glutamate in Chlamydia (psittacosis-trachoma group). J Bacteriol. 1967 Jan;93(1):177–184. doi: 10.1128/jb.93.1.177-184.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weiss E., Wilson N. N., Schramek S., Hill J. C. Partial Reassociation Between the Deoxyribonucleic Acids of Neisseria lactamicus and Neisseria meningitidis. Infect Immun. 1971 Feb;3(2):274–277. doi: 10.1128/iai.3.2.274-277.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES