Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jun;106(3):876–881. doi: 10.1128/jb.106.3.876-881.1971

Fatty Acid Composition of Thermophilic, Mesophilic, and Psychrophilic Clostridia

May Chan 1, Richard H Himes 1, J M Akagi 1
PMCID: PMC248714  PMID: 5567555

Abstract

The fatty acid composition of two thermophilic anaerobes was determined, and the results were compared with those from a mesophilic and a psychrophilic anaerobe. Notable differences were that the thermophiles contained a higher content of saturated straight- and branched-chain fatty acids, and, of the latter, iso C15 was the predominant type. The mesophile and psychrophile were characterized by having a higher percentage of unsaturated fatty acids. An unidentified fatty acid, present in all of the organisms, was purified from the psychrophile. By physical and chemical analysis the structure of the unknown acid was resolved and found to be the unsaturated cyclopropane fatty acid, 12,13-methylene-9-tetradecenoic acid.

Full text

PDF
876

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archibald F. M., Skipski V. P. Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography. J Lipid Res. 1966 May;7(3):442–445. [PubMed] [Google Scholar]
  2. Brian B. L., Gardner E. W. A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Appl Microbiol. 1968 Apr;16(4):549–552. doi: 10.1128/am.16.4.549-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARNAHAN J. E., CASTLE J. E. Some requirements of biological nitrogen fixation. J Bacteriol. 1958 Feb;75(2):121–124. doi: 10.1128/jb.75.2.121-124.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christie W. W., Holman R. T. Mass spectrometry of lipids. I. Cyclopropane fatty acid esters. Lipids. 1966 May;1(3):176–182. doi: 10.1007/BF02531869. [DOI] [PubMed] [Google Scholar]
  5. Daron H. H. Fatty acid composition of lipid extracts of a thermophilic Bacillus species. J Bacteriol. 1970 Jan;101(1):145–151. doi: 10.1128/jb.101.1.145-151.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  7. GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
  8. JAMES A. T. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. doi: 10.1002/9780470110249.ch1. [DOI] [PubMed] [Google Scholar]
  9. MERCER W. A., VAUGHN R. H. The characteristics of some thermophilic, tartrate-fermenting anaerobes. J Bacteriol. 1951 Jul;62(1):27–37. doi: 10.1128/jb.62.1.27-37.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCloskey J. A., Law J. H. Ring location in cyclopropane fatty acid esters by a mass spectrometric method. Lipids. 1967 May;2(3):225–230. doi: 10.1007/BF02532560. [DOI] [PubMed] [Google Scholar]
  11. SCHEURBRANDT G., BLOCH K. Unsaturated fatty acids in microorganisms. J Biol Chem. 1962 Jul;237:2064–2068. [PubMed] [Google Scholar]
  12. SINCLAIR N. A., STOKES J. L. ISOLATION OF OBLIGATELY ANAEROBIC PSYCHROPHILIC BACTERIA. J Bacteriol. 1964 Mar;87:562–565. doi: 10.1128/jb.87.3.562-565.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shen P. Y., Coles E., Foote J. L., Stenesh J. Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus. J Bacteriol. 1970 Aug;103(2):479–481. doi: 10.1128/jb.103.2.479-481.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WILDER M., VALENTINE R. C., AKAGI J. M. FERREDOXIN OF CLOSTRIDIUM THERMOSACCHAROLYTICUM. J Bacteriol. 1963 Oct;86:861–865. doi: 10.1128/jb.86.4.861-865.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yao M., Walker H. W., Lillard D. A. Fatty acids from vegetative cells and spores of Bacillus stearothermophilus. J Bacteriol. 1970 Jun;102(3):877–878. doi: 10.1128/jb.102.3.877-878.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES