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. 1990 Dec;64(12):5750–5756. doi: 10.1128/jvi.64.12.5750-5756.1990

Equine infectious anemia virus derived from a molecular clone persistently infects horses.

L Whetter 1, D Archambault 1, S Perry 1, A Gazit 1, L Coggins 1, A Yaniv 1, D Clabough 1, J Dahlberg 1, F Fuller 1, S Tronick 1
PMCID: PMC248720  PMID: 2173767

Abstract

A full-length molecular clone of equine infectious anemia virus (EIAV) was isolated from a persistently infected canine fetal thymus cell line (Cf2Th). Upon transfection of equine dermis cells, the clone, designated CL22, yielded infectious EIAV particles (CL22-V) that replicated in vitro in both Cf2Th cells and an equine dermis cell strain. Horses infected with CL22-V developed an antibody response to viral proteins and possessed viral DNA in peripheral blood mononuclear cells, as determined by polymerase chain reaction assays. In addition, horses infected with CL22-V became persistently infected and were capable of transmitting the infection by transfer of whole blood to uninfected horses. However, CL22-V, like the parental canine cell-adapted virus, did not cause clinical signs in infected horses. Reverse transcriptase assays of CL22-V- and virulent EIAV-infected equine mononuclear cell cultures indicated that the lack of virulence of CL22-V was not due to an inability to infect and replicate in equine mononuclear cells in vitro.

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Selected References

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