Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jun;106(3):920–924. doi: 10.1128/jb.106.3.920-924.1971

Changes in Hepatic Glycogen, Protein, and Ribonucleic Acid Synthesis, and Some Effects of Cortisol, During Q Fever

J Stueckemann 1, D Paretsky 1
PMCID: PMC248726  PMID: 5557597

Abstract

Liver glycogen is depleted in guinea pigs infected with Coxiella burneti. Syntheses of the glycogen precursors uridine triphosphate and uridine diphosphate glucose are unaffected during Q fever, but glycogen synthetase activity is inhibited. Exogenous cortisol relieves this inhibition in infected animals. Orotate and amino acids are more rapidly incorporated into ribonucleic acid and protein during infection. It is proposed that the biochemical defect in the synthesis of glycogen lies in the inactivation of glycogen synthetase.

Full text

PDF
920

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BREZINA R., SCHRAMEK S., URVOELGYI J. STUDY OF THE ANTIGENIC STRUCTURE OF COXIELLA BURNETI. 3. PYROGENIC EFFECT OF PHASE I ANTIGEN IN EXPERIMENTAL GUINEA PIGS. Acta Virol. 1965 Mar;9:180–185. [PubMed] [Google Scholar]
  2. Berry L. J., Smythe D. S., Colwell L. S. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J Bacteriol. 1966 Jul;92(1):107–115. doi: 10.1128/jb.92.1.107-115.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bucher N. L., Swaffield M. N. Rate of incorporation of [6-14C]orotic acid into uridine 5'-triphosphate and cytidine 5'-triphosphate and nuclear ribonucleic acid in regenerating rat liver. Biochim Biophys Acta. 1965 Dec 9;108(4):551–567. doi: 10.1016/0005-2787(65)90052-3. [DOI] [PubMed] [Google Scholar]
  4. De Wulf H., Hers H. G. The stimulation of glycogen synthesis andof glycogen synthetase in the liver by glucocorticoids. Eur J Biochem. 1967 Jul;2(1):57–60. doi: 10.1111/j.1432-1033.1967.tb00105.x. [DOI] [PubMed] [Google Scholar]
  5. Drews J., Brawerman G. Alterations in the nature of ribonucleic acid synthesized in rat liver during regeneration and after cortisol administration. J Biol Chem. 1967 Mar 10;242(5):801–808. [PubMed] [Google Scholar]
  6. Feigelson M., Feigelson P. Relationships between hepatic enzyme induction, glutamate formation, and purine nucleotide biosynthesis in glucocorticoid action. J Biol Chem. 1966 Dec 25;241(24):5819–5826. [PubMed] [Google Scholar]
  7. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOAGLAND M. B. Some factors influencing protein synthetic activity in a cell-free mammalian system. Cold Spring Harb Symp Quant Biol. 1961;26:153–157. doi: 10.1101/sqb.1961.026.01.019. [DOI] [PubMed] [Google Scholar]
  9. Hishizawa T., Terayama H. Incorporation of amino acids by cell-free systems from regenerating rat liver. J Biochem. 1966 Mar;59(3):272–279. [PubMed] [Google Scholar]
  10. Hornbrook K. R., Burch H. B., Lowry O. H. The effects of adrenalectomy and hydrocortisone on rat liver metabolites and glycogen synthetase activity. Mol Pharmacol. 1966 Mar;2(2):106–116. [PubMed] [Google Scholar]
  11. KAHAN J. A rapid photometric method for the determination of glycogen. Arch Biochem Biophys. 1953 Dec;47(2):408–418. doi: 10.1016/0003-9861(53)90477-9. [DOI] [PubMed] [Google Scholar]
  12. LELOIR L. F., OLAVARRIA J. M., GOLDEMBERG S. H., CARMINATTI H. Biosynthesis of glycogen from uridine diphosphate glucose. Arch Biochem Biophys. 1959 Apr;81(2):508–520. doi: 10.1016/0003-9861(59)90232-2. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. MERCADO T. I., VON BRAND T. Glycogen studies on white rats infected with Plasmodium berghei. Exp Parasitol. 1954 May;3(3):259–266. doi: 10.1016/0014-4894(54)90025-9. [DOI] [PubMed] [Google Scholar]
  15. MUNCH-PETERSEN A., KALCKAR H. M., CUTOLO E., SMITH E. E. Uridyl transferases and the formation of uridine triphosphate; enzymic production of uridine triphosphate: uridine diphosphoglucose pyrophosphorolysis. Nature. 1953 Dec 5;172(4388):1036–1037. doi: 10.1038/1721036a0. [DOI] [PubMed] [Google Scholar]
  16. Mallavia L. P., Paretsky D. Physiology of rickettsiae. VII. Amino acid incorporation by Coxiella burnetii and by infected hosts. J Bacteriol. 1967 May;93(5):1479–1483. doi: 10.1128/jb.93.5.1479-1483.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mirone L. Effect of prolonged ethanol intake on body weight, liver weight and liver nitrogen, glycogen, ADH, NAD and NADH of mice. Life Sci. 1965 Jun;4(12):1195–1199. doi: 10.1016/0024-3205(65)90332-2. [DOI] [PubMed] [Google Scholar]
  18. Munro H. N., Fleck A. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review. Analyst. 1966 Feb;91(79):78–88. doi: 10.1039/an9669100078. [DOI] [PubMed] [Google Scholar]
  19. PARETSKY D., DOWNS C. M., SALMON C. W. SOME BIOCHEMICAL CHANGES IN THE GUINEA PIG DURING INFECTION WITH COXIELLA BURNETII. J Bacteriol. 1964 Jul;88:137–142. doi: 10.1128/jb.88.1.137-142.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PITOT H. C., PERAINO C. STUDIES ON THE INDUCTION AND REPRESSION OF ENZYMES IN RAT LIVER. I. INDUCTION OF THREONINE DEHYDRASE AND ORNITHINE-DELTA-TRANSAMINASE BY ORAL INTUBATION OF CASEIN HYDROLYSATE. J Biol Chem. 1964 Jun;239:1783–1788. [PubMed] [Google Scholar]
  21. Paretsky D., Stueckemann J. Chemical and biochemical changes in subcellular fractions of guinea pig liver during infection with Coxiella burneti. J Bacteriol. 1970 May;102(2):334–340. doi: 10.1128/jb.102.2.334-340.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SIE H. G., FISHMAN W. H. GLYCOGEN SYNTHETASE: ITS RESPONSE TO CORTISOL. Science. 1964 Feb 21;143(3608):816–817. doi: 10.1126/science.143.3608.816. [DOI] [PubMed] [Google Scholar]
  23. SINGH V. N., VENKITASUBRAMANIAN T. A., VISWANATHAN R. Study of glucose tolerance and synthesis of hepatic glycogen from glucose and glycine-1-C-14 in tuberculous guinea pigs. Arch Biochem Biophys. 1963 May;101:229–233. doi: 10.1016/s0003-9861(63)80007-7. [DOI] [PubMed] [Google Scholar]
  24. SMITH E. E., MILLS G. T. Uridine nucleotide compounds of liver. Biochim Biophys Acta. 1954 Mar;13(3):386–400. doi: 10.1016/0006-3002(54)90346-7. [DOI] [PubMed] [Google Scholar]
  25. Tata J. R. Hormonal regulation of growth and protein synthesis. Nature. 1968 Jul 27;219(5152):331–337. doi: 10.1038/219331a0. [DOI] [PubMed] [Google Scholar]
  26. de Wulf H., Hers H. G. The role of glucose, glucagon and glucocorticoids in the regulation of liver glycogen synthesis. Eur J Biochem. 1968 Dec 5;6(4):558–564. doi: 10.1111/j.1432-1033.1968.tb00481.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES