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. 1971 Jun;106(3):931–937. doi: 10.1128/jb.106.3.931-937.1971

Modification of the Composition and Structure of the Yeast Cell Wall by Culture in the Presence of Sulfur Amino Acids

K A Killick 1
PMCID: PMC248729  PMID: 4934069

Abstract

Growth of Candida utilis and Saccharomyces cerevisiae in a medium supplemented with sulfur amino acids led to synthesis and accumulation of S-adenosylmethionine, accompanied by a reduction in the cell yield, an increased sensitivity of the cell wall to snail gut enzymes (Helix pomatia), as judged by spheroplast formation, and by a modification of the chemical composition of both the intact cells and their isolated walls. Walls of supplemented cultures of C. utilis were three times as sensitive to enzymatic digestion as walls from nonsupplemented cultures. In contrast to C. utilis, walls isolated from supplemented cultures of S. cerevisiae were digested slightly more rapidly by the purified snail extract than those from nonsupplemented cultures. Chemical modifications of the cell wall are interpreted to explain the ease with which cells from sulfur amino acid-supplemented cultures are converted to spheroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTNICKI-GARCIA S., NICKERSON W. J. Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii. Biochim Biophys Acta. 1962 Mar 26;58:102–119. doi: 10.1016/0006-3002(62)90822-3. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown C. M., Rose A. H. Effects of temperature on composition and cell volume of Candida utilis. J Bacteriol. 1969 Jan;97(1):261–270. doi: 10.1128/jb.97.1.261-272.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHUNG C. W., NICKERSON W. J. Polysaccharide syntheses in growing yeasts. J Biol Chem. 1954 May;208(1):395–407. [PubMed] [Google Scholar]
  5. DUELL E. A., INOUE S., UTTER M. F. ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST. J Bacteriol. 1964 Dec;88:1762–1773. doi: 10.1128/jb.88.6.1762-1773.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GHOSH A., CHARALAMPOUS F., SISON Y., BORER R. Metabolic function of myo-inositol. I. Cytological and chemical alterations in yeast resulting from inositol deficiency. J Biol Chem. 1960 Sep;235:2522–2528. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MILLBANK J. W., MACRAE R. M. DEGRADATION OF YEAST CELL WALL BY FRACTIONATED SNAIL GUT ENZYME. Nature. 1964 Mar 28;201:1347–1347. doi: 10.1038/2011347a0. [DOI] [PubMed] [Google Scholar]
  9. McLellan W. L., Jr, Lampen J. O. Phosphomannanase (PR-factor), an enzyme required for the formation of yeast protoplasts. J Bacteriol. 1968 Mar;95(3):967–974. doi: 10.1128/jb.95.3.967-974.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McMurrough I., Rose A. H. Effect of growth rate and substrate limitation on the composition and structure of the cell wall of Saccharomyces cerevisiae. Biochem J. 1967 Oct;105(1):189–203. doi: 10.1042/bj1050189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NICKERSON W. J. SYMPOSIUM ON BIOCHEMICAL BASES OF MORPHOGENESIS IN FUNGI. IV. MOLECULAR BASES OF FORM IN YEASTS. Bacteriol Rev. 1963 Sep;27:305–324. doi: 10.1128/br.27.3.305-324.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OGUR M., ROSEN G. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch Biochem. 1950 Feb;25(2):262–276. [PubMed] [Google Scholar]
  13. PHAFF H. J. CELL WALL OF YEASTS. Annu Rev Microbiol. 1963;17:15–30. doi: 10.1146/annurev.mi.17.100163.000311. [DOI] [PubMed] [Google Scholar]
  14. RONDLE C. J., MORGAN W. T. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. doi: 10.1042/bj0610586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]
  16. SCHLENK F., DAINKO J. L., STANFORD S. M. Improved procedure for the isolation of S-adenosylmethionine and S-adenosylethionine. Arch Biochem Biophys. 1959 Jul;83(1):28–34. doi: 10.1016/0003-9861(59)90006-2. [DOI] [PubMed] [Google Scholar]
  17. SVIHLA G., SCHLENK F., DAINKO J. L. Spheroplasts of the yeast Candida utilis. J Bacteriol. 1961 Dec;82:808–814. doi: 10.1128/jb.82.6.808-814.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

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