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. 1991 Sep;65(9):5061–5067. doi: 10.1128/jvi.65.9.5061-5067.1991

Isolation, identification, and partial cDNA cloning of genomic RNA of jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis.

D F York 1, R Vigne 1, D W Verwoerd 1, G Querat 1
PMCID: PMC248970  PMID: 1651422

Abstract

The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis (jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and characterized. It has an insert of 2.1 kb which hybridizes to the same 8.7-kb RNA in all the JSRV-infected sheep lung washes tested but does not hybridize to maedi-visna virus, a sheep lentivirus often found coinfecting JSRV-infected lungs. Comparison of the amino acid sequence encoded by JS 46.1 with those encoded by other retroviruses revealed that JSRV has homology to the type D and B oncoviruses and to human endogenous retrovirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chiu I. M., Callahan R., Tronick S. R., Schlom J., Aaronson S. A. Major pol gene progenitors in the evolution of oncoviruses. Science. 1984 Jan 27;223(4634):364–370. doi: 10.1126/science.6197754. [DOI] [PubMed] [Google Scholar]
  2. DeMartini J. C., Rosadio R. H., Sharp J. M., Russell H. I., Lairmore M. D. Experimental coinduction of type D retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs. J Natl Cancer Inst. 1987 Jul;79(1):167–177. [PubMed] [Google Scholar]
  3. Demartini J. C., Rosadio R. H., Lairmore M. D. The etiology and pathogenesis of ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Microbiol. 1988 Jul;17(3):219–236. doi: 10.1016/0378-1135(88)90067-3. [DOI] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Herring A. J., Sharp J. M., Scott F. M., Angus K. W. Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte). Vet Microbiol. 1983 Jun;8(3):237–249. doi: 10.1016/0378-1135(83)90076-7. [DOI] [PubMed] [Google Scholar]
  7. Kanehisa M. I. Los Alamos sequence analysis package for nucleic acids and proteins. Nucleic Acids Res. 1982 Jan 11;10(1):183–196. doi: 10.1093/nar/10.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kanehisa M. Use of statistical criteria for screening potential homologies in nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):203–213. doi: 10.1093/nar/12.1part1.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lairmore M. D., Rosadio R. H., DeMartini J. C. Ovine lentivirus lymphoid interstitial pneumonia. Rapid induction in neonatal lambs. Am J Pathol. 1986 Oct;125(1):173–181. [PMC free article] [PubMed] [Google Scholar]
  11. Lairmore M. D., Rosadio R. H., DeMartini J. C. Ovine lentivirus lymphoid interstitial pneumonia. Rapid induction in neonatal lambs. Am J Pathol. 1986 Oct;125(1):173–181. [PMC free article] [PubMed] [Google Scholar]
  12. Martin W. B., Scott F. M., Sharp J. M., Angus K. W., Norval M. Experimental production of sheep pulmonary adenomatosis (Jaagsiekte). Nature. 1976 Nov 11;264(5582):183–185. doi: 10.1038/264183a0. [DOI] [PubMed] [Google Scholar]
  13. McClure M. A., Johnson M. S., Feng D. F., Doolittle R. F. Sequence comparisons of retroviral proteins: relative rates of change and general phylogeny. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2469–2473. doi: 10.1073/pnas.85.8.2469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moore R., Dixon M., Smith R., Peters G., Dickson C. Complete nucleotide sequence of a milk-transmitted mouse mammary tumor virus: two frameshift suppression events are required for translation of gag and pol. J Virol. 1987 Feb;61(2):480–490. doi: 10.1128/jvi.61.2.480-490.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ono M., Yasunaga T., Miyata T., Ushikubo H. Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome. J Virol. 1986 Nov;60(2):589–598. doi: 10.1128/jvi.60.2.589-598.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Payne A. L., Verwoerd D. W., Garnett H. M. The morphology and morphogenesis of jaagsiekte retrovirus (JSRV). Onderstepoort J Vet Res. 1983 Dec;50(4):317–322. [PubMed] [Google Scholar]
  17. Payne A., York D. F., de Villiers E. M., Verwoerd D. W., Quérat G., Barban V., Sauze N., Vigne R. Isolation and identification of a South African lentivirus from jaagsiekte lungs. Onderstepoort J Vet Res. 1986 Mar;53(1):55–62. [PubMed] [Google Scholar]
  18. Perk K., Hod I. Sheep lung carcinoma: an endemic analogue of a sporadic human neoplasm. J Natl Cancer Inst. 1982 Oct;69(4):747–749. [PubMed] [Google Scholar]
  19. Perk K., Michalides R., Spiegelman S., Schlom J. Biochemical and morphologic evidence for the presence of an RNA tumor virus in pulmonary carcinoma of sheep (Jaagsiekte). J Natl Cancer Inst. 1974 Jul;53(1):131–135. doi: 10.1093/jnci/53.1.131. [DOI] [PubMed] [Google Scholar]
  20. Perk K., Yaniv A. Lack of maedi viral related RNA in pulmonary carcinoma of sheep (jaagsiekte). Res Vet Sci. 1978 Jan;24(1):46–48. [PubMed] [Google Scholar]
  21. Power M. D., Marx P. A., Bryant M. L., Gardner M. B., Barr P. J., Luciw P. A. Nucleotide sequence of SRV-1, a type D simian acquired immune deficiency syndrome retrovirus. Science. 1986 Mar 28;231(4745):1567–1572. doi: 10.1126/science.3006247. [DOI] [PubMed] [Google Scholar]
  22. Querat G., Audoly G., Sonigo P., Vigne R. Nucleotide sequence analysis of SA-OMVV, a visna-related ovine lentivirus: phylogenetic history of lentiviruses. Virology. 1990 Apr;175(2):434–447. doi: 10.1016/0042-6822(90)90428-t. [DOI] [PubMed] [Google Scholar]
  23. Quérat G., Barban V., Sauze N., Vigne R., Payne A., York D., De Villiers E. M., Verwoerd D. W. Characteristics of a novel lentivirus derived from South African sheep with pulmonary adenocarcinoma (jaagsiekte). Virology. 1987 May;158(1):158–167. doi: 10.1016/0042-6822(87)90249-2. [DOI] [PubMed] [Google Scholar]
  24. Rosadio R. H., Sharp J. M., Lairmore M. D., Dahlberg J. E., De Martini J. C. Lesions and retroviruses associated with naturally occurring ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Pathol. 1988 Jan;25(1):58–66. doi: 10.1177/030098588802500108. [DOI] [PubMed] [Google Scholar]
  25. Sagata N., Yasunaga T., Tsuzuku-Kawamura J., Ohishi K., Ogawa Y., Ikawa Y. Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses. Proc Natl Acad Sci U S A. 1985 Feb;82(3):677–681. doi: 10.1073/pnas.82.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sharp J. M., Angus K. W., Gray E. W., Scott F. M. Rapid transmission of sheep pulmonary adenomatosis (jaagsiekte) in young lambs. Brief report. Arch Virol. 1983;78(1-2):89–95. doi: 10.1007/BF01310861. [DOI] [PubMed] [Google Scholar]
  27. Sharp J. M., Angus K. W., Jassim F. A., Scott F. M. Experimental transmission of sheep pulmonary adenomatosis to a goat. Vet Rec. 1986 Sep 6;119(10):245–245. doi: 10.1136/vr.119.10.245. [DOI] [PubMed] [Google Scholar]
  28. Snyder S. P., DeMartini J. C., Ameghino E., Caletti E. Coexistence of pulmonary adenomatosis and progressive pneumonia in sheep in the central sierra of Peru. Am J Vet Res. 1983 Jul;44(7):1334–1338. [PubMed] [Google Scholar]
  29. Sonigo P., Barker C., Hunter E., Wain-Hobson S. Nucleotide sequence of Mason-Pfizer monkey virus: an immunosuppressive D-type retrovirus. Cell. 1986 May 9;45(3):375–385. doi: 10.1016/0092-8674(86)90323-5. [DOI] [PubMed] [Google Scholar]
  30. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tustin R. C., Williamson A. L., York D. F., Verwoerd D. W. Experimental transmission of jaagsiekte (ovine pulmonary adenomatosis) to goats. Onderstepoort J Vet Res. 1988 Mar;55(1):27–32. [PubMed] [Google Scholar]
  32. Verwoerd D. W., Payne A. L., York D. F., Myer M. S. Isolation and preliminary characterization of the jaagsiekte retrovirus (JSRV). Onderstepoort J Vet Res. 1983 Dec;50(4):309–316. [PubMed] [Google Scholar]
  33. Verwoerd D. W., Williamson A. L., De Villiers E. M. Aetiology of jaagsiekte: transmission by means of subcellular fractions and evidence for the involvement of a retrovirus. Onderstepoort J Vet Res. 1980 Dec;47(4):275–280. [PubMed] [Google Scholar]
  34. Vigne R., Barban V., Quérat G., Mazarin V., Gourdou I., Sauze N. Transcription of visna virus during its lytic cycle: evidence for a sequential early and late gene expression. Virology. 1987 Nov;161(1):218–227. doi: 10.1016/0042-6822(87)90188-7. [DOI] [PubMed] [Google Scholar]
  35. Vigne R., Filippi P., Quérat G., Sauze N., Vitu C., Russo P., Delori P. Precursor polypeptides to structural proteins of visna virus. J Virol. 1982 Jun;42(3):1046–1056. doi: 10.1128/jvi.42.3.1046-1056.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wandera J. G. Sheep pulmonary adenomatosis (Jaagsiekte). Adv Vet Sci Comp Med. 1971;15:251–283. [PubMed] [Google Scholar]
  37. York D. F., Williamson A., Barnard B. J., Verwoerd D. W. Some characteristics of a retrovirus isolated from transformed bovine cells. Virology. 1989 Aug;171(2):394–400. doi: 10.1016/0042-6822(89)90607-7. [DOI] [PubMed] [Google Scholar]

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