Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1991 Oct;65(10):5244–5249. doi: 10.1128/jvi.65.10.5244-5249.1991

Predominant binding of Theiler's viruses to a 34-kilodalton receptor protein on susceptible cell lines.

D R Kilpatrick 1, H L Lipton 1
PMCID: PMC249003  PMID: 1895381

Abstract

Western immunoblots of BHK-21 cell lysates probed with the highly virulent GDVII and the less virulent BeAn strains of Theiler's murine encephalomyelitis virus (TMEV) revealed predominant binding to a 34-kDa membrane protein and much lower levels of binding to 100- and 18-kDa membrane proteins. Complete inhibition of virus binding to both the 34- and 18-kDa membrane species by excess unlabeled TMEV demonstrated specificity of binding. Virus binding was also blocked by wheat germ agglutinin, which specifically binds to sialic acid residues and blocks TMEV binding to whole BHK-21 cells. Radiolabeled TMEV also bound to 100-, 34-, and 18-kDa membrane proteins expressed on other TMEV permissive cell lines but not on the nonpermissive cell lines tested. These data suggest that a 34-kDa cellular protein may be the primary determinant of susceptibility to TMEV infection by mediating the binding of GDVII and BeAn viruses to susceptible cells.

Full text

PDF
5244

Images in this article

5245Image
on p.5245
5246Image
on p.5246
5246Image
on p.5246
5246Image
on p.5246
5247Image
on p.5247
5247Image
on p.5247
5248Image
on p.5248
5248Image
on p.5248

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adlish J. D., Lahijani R. S., St Jeor S. C. Identification of a putative cell receptor for human cytomegalovirus. Virology. 1990 Jun;176(2):337–345. doi: 10.1016/0042-6822(90)90003-a. [DOI] [PubMed] [Google Scholar]
  2. Atreya C. D., Raccah B., Pirone T. P. A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology. 1990 Sep;178(1):161–165. doi: 10.1016/0042-6822(90)90389-9. [DOI] [PubMed] [Google Scholar]
  3. Bae Y. S., Eun H. M., Pon R. T., Giron D., Yoon J. W. Two amino acids, Phe 16 and Ala 776, on the polyprotein are most likely to be responsible for the diabetogenicity of encephalomyocarditis virus. J Gen Virol. 1990 Mar;71(Pt 3):639–645. doi: 10.1099/0022-1317-71-3-639. [DOI] [PubMed] [Google Scholar]
  4. Boyle J. F., Weismiller D. G., Holmes K. V. Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues. J Virol. 1987 Jan;61(1):185–189. doi: 10.1128/jvi.61.1.185-189.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burness A. T., Pardoe I. U. A sialoglycopeptide from human erythrocytes with receptor-like properties for encephalomyocarditis and influenza viruses. J Gen Virol. 1983 May;64(Pt 5):1137–1148. doi: 10.1099/0022-1317-64-5-1137. [DOI] [PubMed] [Google Scholar]
  6. Calenoff M. A., Faaberg K. S., Lipton H. L. Genomic regions of neurovirulence and attenuation in Theiler murine encephalomyelitis virus. Proc Natl Acad Sci U S A. 1990 Feb;87(3):978–982. doi: 10.1073/pnas.87.3.978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cordonnier A., Montagnier L., Emerman M. Single amino-acid changes in HIV envelope affect viral tropism and receptor binding. Nature. 1989 Aug 17;340(6234):571–574. doi: 10.1038/340571a0. [DOI] [PubMed] [Google Scholar]
  8. Dubensky T. W., Freund R., Dawe C. J., Benjamin T. L. Polyomavirus replication in mice: influences of VP1 type and route of inoculation. J Virol. 1991 Jan;65(1):342–349. doi: 10.1128/jvi.65.1.342-349.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Epstein R. L., Powers M. L., Rogart R. B., Weiner H. L. Binding of 125I-labeled reovirus to cell surface receptors. Virology. 1984 Feb;133(1):46–55. doi: 10.1016/0042-6822(84)90424-0. [DOI] [PubMed] [Google Scholar]
  10. Fotiadis C., Kilpatrick D. R., Lipton H. L. Comparison of the binding characteristics to BHK-21 cells of viruses representing the two Theiler's virus neurovirulence groups. Virology. 1991 May;182(1):365–370. doi: 10.1016/0042-6822(91)90683-3. [DOI] [PubMed] [Google Scholar]
  11. Fu J. L., Stein S., Rosenstein L., Bodwell T., Routbort M., Semler B. L., Roos R. P. Neurovirulence determinants of genetically engineered Theiler viruses. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4125–4129. doi: 10.1073/pnas.87.11.4125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gershoni J. M., Lapidot M., Zakai N., Loyter A. Protein blot analysis of virus receptors: identification and characterization of the Sendai virus receptor. Biochim Biophys Acta. 1986 Mar 27;856(1):19–26. doi: 10.1016/0005-2736(86)90004-0. [DOI] [PubMed] [Google Scholar]
  13. Kilpatrick D. R., Srinivas R. V., Compans R. W. The spleen focus-forming virus envelope glycoprotein is defective in oligomerization. J Biol Chem. 1989 Jun 25;264(18):10732–10737. [PubMed] [Google Scholar]
  14. Kilpatrick D. R., Srinivas R. V., Stephens E. B., Compans R. W. Effects of deletion of the cytoplasmic domain upon surface expression and membrane stability of a viral envelope glycoprotein. J Biol Chem. 1987 Nov 25;262(33):16116–16121. [PubMed] [Google Scholar]
  15. Krishnaswamy S., Rossmann M. G. Structural refinement and analysis of Mengo virus. J Mol Biol. 1990 Feb 20;211(4):803–844. doi: 10.1016/0022-2836(90)90077-Y. [DOI] [PubMed] [Google Scholar]
  16. Lipton H. L., Calenoff M., Bandyopadhyay P., Miller S. D., Dal Canto M. C., Gerety S., Jensen K. The 5' noncoding sequences from a less virulent Theiler's virus dramatically attenuate GDVII neurovirulence. J Virol. 1991 Aug;65(8):4370–4377. doi: 10.1128/jvi.65.8.4370-4377.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lipton H. L. Characterization of the TO strains of Theiler's mouse encephalomyelitis viruses. Infect Immun. 1978 Jun;20(3):869–872. doi: 10.1128/iai.20.3.869-872.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Liu C., Collins J., Sharp E. The pathogenesis of Theiler's GD VII encephalomyelitis virus infection in mice as studied by immunofluorescent technique and infectivity titrations. J Immunol. 1967 Jan;98(1):46–55. [PubMed] [Google Scholar]
  19. Luo M., Vriend G., Kamer G., Minor I., Arnold E., Rossmann M. G., Boege U., Scraba D. G., Duke G. M., Palmenberg A. C. The atomic structure of Mengo virus at 3.0 A resolution. Science. 1987 Jan 9;235(4785):182–191. doi: 10.1126/science.3026048. [DOI] [PubMed] [Google Scholar]
  20. McAllister A., Tangy F., Aubert C., Brahic M. Genetic mapping of the ability of Theiler's virus to persist and demyelinate. J Virol. 1990 Sep;64(9):4252–4257. doi: 10.1128/jvi.64.9.4252-4257.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miller S. D., Clatch R. J., Pevear D. C., Trotter J. L., Lipton H. L. Class II-restricted T cell responses in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease. I. Cross-specificity among TMEV substrains and related picornaviruses, but not myelin proteins. J Immunol. 1987 Jun 1;138(11):3776–3784. [PubMed] [Google Scholar]
  22. Ohara Y., Roos R. The antibody response in Theiler's virus infection: new perspectives on multiple sclerosis. Prog Med Virol. 1987;34:156–179. [PubMed] [Google Scholar]
  23. Pevear D. C., Luo M., Lipton H. L. Three-dimensional model of the capsid proteins of two biologically different Theiler virus strains: clustering of amino acid difference identifies possible locations of immunogenic sites on the virion. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4496–4500. doi: 10.1073/pnas.85.12.4496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rogers G. N., Pritchett T. J., Lane J. L., Paulson J. C. Differential sensitivity of human, avian, and equine influenza A viruses to a glycoprotein inhibitor of infection: selection of receptor specific variants. Virology. 1983 Dec;131(2):394–408. doi: 10.1016/0042-6822(83)90507-x. [DOI] [PubMed] [Google Scholar]
  25. Rozhon E. J., Kratochvil J. D., Lipton H. L. Analysis of genetic variation in Theiler's virus during persistent infection in the mouse central nervous system. Virology. 1983 Jul 15;128(1):16–32. doi: 10.1016/0042-6822(83)90315-x. [DOI] [PubMed] [Google Scholar]
  26. Sethi P., Lipton H. L. The growth of four human and animal enteroviruses in the central nervous systems of mice. J Neuropathol Exp Neurol. 1981 May;40(3):258–270. doi: 10.1097/00005072-198105000-00004. [DOI] [PubMed] [Google Scholar]
  27. Shioda T., Levy J. A., Cheng-Mayer C. Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp120 gene. Nature. 1991 Jan 10;349(6305):167–169. doi: 10.1038/349167a0. [DOI] [PubMed] [Google Scholar]
  28. Tangy F., McAllister A., Aubert C., Brahic M. Determinants of persistence and demyelination of the DA strain of Theiler's virus are found only in the VP1 gene. J Virol. 1991 Mar;65(3):1616–1618. doi: 10.1128/jvi.65.3.1616-1618.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Taylor H. P., Cooper N. R. The human cytomegalovirus receptor on fibroblasts is a 30-kilodalton membrane protein. J Virol. 1990 Jun;64(6):2484–2490. doi: 10.1128/jvi.64.6.2484-2490.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tomassini J. E., Graham D., DeWitt C. M., Lineberger D. W., Rodkey J. A., Colonno R. J. cDNA cloning reveals that the major group rhinovirus receptor on HeLa cells is intercellular adhesion molecule 1. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4907–4911. doi: 10.1073/pnas.86.13.4907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Uncapher C. R., DeWitt C. M., Colonno R. J. The major and minor group receptor families contain all but one human rhinovirus serotype. Virology. 1991 Feb;180(2):814–817. doi: 10.1016/0042-6822(91)90098-v. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES