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. 1991 Oct;65(10):5499–5505. doi: 10.1128/jvi.65.10.5499-5505.1991

Avian reovirus S1133 can replicate in mouse L cells: effect of pH and cell attachment status on viral infection.

M Mallo 1, J Martínez-Costas 1, J Benavente 1
PMCID: PMC249045  PMID: 1895398

Abstract

Previous reports have suggested that avian reovirus S1133 fails to replicate in mouse L cells. In this article, we report that replication does occur under certain culture conditions. The avian reovirus was found to grow in mouse L cells at pH 6.4 and 7.2 but not at pH 8.2. Culture medium with a basic pH directly inhibited viral transcription and genome replication. As a result, viral protein synthesis was also affected. At permissive pH levels, avian reovirus grew better in monolayers than in suspension cultures of L cells because of the influence of cell attachment status on viral macromolecular synthesis. Our results not only show that avian reovirus can replicate in mouse L cells but also help to explain why it did not in previous studies.

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Selected References

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