Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1991 Oct;65(10):5506–5512. doi: 10.1128/jvi.65.10.5506-5512.1991

The stimulatory effect of actinomycin D on avian reovirus replication in L cells suggests that translational competition dictates the fate of the infection.

M Mallo 1, J Martínez-Costas 1, J Benavente 1
PMCID: PMC249046  PMID: 1895399

Abstract

Indirect immunostaining of avian reovirus S1133-infected L-cell monolayers showed that most of the cells can support viral replication. However, the number of cells in which the virus was actually replicating depended on the multiplicity of virus infection. The presence of actinomycin D during infection increased viral protein synthesis, viral growth, and the number of actively infected cells at late infection times. The antibiotic elicited these effects by triggering viral replication in cells that already contained unproductive cytoplasmic virus but that would not get productively infected in the absence of the drug. From these results, we propose a model for the interaction between L cells and avian reovirus S1133 in which viral versus host mRNA competition for the translational machinery determines the fate of the virus infection.

Full text

PDF
5506

Images in this article

5507Image
on p.5507
5508Image
on p.5508
5509Image
on p.5509
5510Image
on p.5510

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam S. A., Choi Y. D., Dreyfuss G. Interaction of mRNA with proteins in vesicular stomatitis virus-infected cells. J Virol. 1986 Feb;57(2):614–622. doi: 10.1128/jvi.57.2.614-622.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benavente J., Shatkin A. J. Avian reovirus mRNAs are nonfunctional in infected mouse cells: translational basis for virus host-range restriction. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4257–4261. doi: 10.1073/pnas.85.12.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brendler T., Godefroy-Colburn T., Carlill R. D., Thach R. E. The role of mRNA competition in regulating translation. II. Development of a quantitative in vitro assay. J Biol Chem. 1981 Nov 25;256(22):11747–11754. [PubMed] [Google Scholar]
  4. Brendler T., Godefroy-Colburn T., Yu S., Thach R. E. The role of mRNA competition in regulating translation. III. Comparison of in vitro and in vivo results. J Biol Chem. 1981 Nov 25;256(22):11755–11761. [PubMed] [Google Scholar]
  5. GOMATOS P. J., TAMM I., DALES S., FRANKLIN R. M. Reovirus type 3: physical characteristics and interaction with L cells. Virology. 1962 Jul;17:441–454. doi: 10.1016/0042-6822(62)90139-3. [DOI] [PubMed] [Google Scholar]
  6. Glass S. E., Naqi S. A., Hall C. F., Kerr K. M. Isolation and characterization of a virus associated with arthritis of chickens. Avian Dis. 1973 Apr-Jun;17(2):415–424. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Mallo M., Martínez-Costas J., Benavente J. Avian reovirus S1133 can replicate in mouse L cells: effect of pH and cell attachment status on viral infection. J Virol. 1991 Oct;65(10):5499–5505. doi: 10.1128/jvi.65.10.5499-5505.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Neill R. E., Racaniello V. R. Inhibition of translation in cells infected with a poliovirus 2Apro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 2. J Virol. 1989 Dec;63(12):5069–5075. doi: 10.1128/jvi.63.12.5069-5075.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ray A., Walden W. E., Brendler T., Zenger V. E., Thach R. E. Effect of medium hypertonicity on reovirus translation rates. An application of kinetic modeling in vivo. Biochemistry. 1985 Dec 17;24(26):7525–7532. doi: 10.1021/bi00347a004. [DOI] [PubMed] [Google Scholar]
  11. SHATKIN A. J. ACTINOMYCIN AND THE DIFFERENTIAL SYNTHESIS OF REOVIRUS AND L CELL RNA. Biochem Biophys Res Commun. 1965 May 3;19:506–510. doi: 10.1016/0006-291x(65)90154-3. [DOI] [PubMed] [Google Scholar]
  12. Schnitzer T. J., Ramos T., Gouvea V. Avian reovirus polypeptides: analysis of intracellular virus-specified products, virions, top component, and cores. J Virol. 1982 Sep;43(3):1006–1014. doi: 10.1128/jvi.43.3.1006-1014.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sharpe A. H., Fields B. N. Reovirus inhibition of cellular RNA and protein synthesis: role of the S4 gene. Virology. 1982 Oct 30;122(2):381–391. doi: 10.1016/0042-6822(82)90237-9. [DOI] [PubMed] [Google Scholar]
  14. Silverstein S. C., Astell C., Levin D. H., Schonberg M., Acs G. The mechanisms of reovirus uncoating and gene activation in vivo. Virology. 1972 Mar;47(3):797–806. doi: 10.1016/0042-6822(72)90571-5. [DOI] [PubMed] [Google Scholar]
  15. Spandidos D. A., Graham A. F. Nonpermissive infection of L cells by an avian reovirus: restricted transcription of the viral genome. J Virol. 1976 Sep;19(3):977–984. doi: 10.1128/jvi.19.3.977-984.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spandidos D. A., Graham A. F. Physical and chemical characterization of an avian reovirus. J Virol. 1976 Sep;19(3):968–976. doi: 10.1128/jvi.19.3.968-976.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stacy-Phipps S., Patton J. T. Synthesis of plus- and minus-strand RNA in rotavirus-infected cells. J Virol. 1987 Nov;61(11):3479–3484. doi: 10.1128/jvi.61.11.3479-3484.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Van der Heide L., Geissler J., Bryant E. S. Infectious tenosynovitis: serologic and histopathologic response after experimental infection with a Connecticut isolate. Avian Dis. 1974 Jul;18(3):289–296. [PubMed] [Google Scholar]
  19. Walden W. E., Godefroy-Colburn T., Thach R. E. The role of mRNA competition in regulating translation. I. Demonstration of competition in vivo. J Biol Chem. 1981 Nov 25;256(22):11739–11746. [PubMed] [Google Scholar]
  20. Weiss B., Nitschko H., Ghattas I., Wright R., Schlesinger S. Evidence for specificity in the encapsidation of Sindbis virus RNAs. J Virol. 1989 Dec;63(12):5310–5318. doi: 10.1128/jvi.63.12.5310-5318.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES