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. 1991 Oct;65(10):5535–5538. doi: 10.1128/jvi.65.10.5535-5538.1991

Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK.

M E Hardy 1, G N Woode 1, Z C Xu 1, M Gorziglia 1
PMCID: PMC249053  PMID: 1654450

Abstract

In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid sequences were deduced and compared with those of NCDV and bovine rotavirus strain UK. Amino acid sequence homology in VP7 between the three strains was greater than 94%, confirming their relationship as VP7 serotype 6 viruses. VP4 of B641 showed amino acid homology to UK of 94% but only 73% homology to NCDV. Sequence comparison of a variable region of VP8 demonstrated amino acid homology of 53% between B641 and NCDV, whereas B641 and UK were 89% homologous in this region. These results confirm the earlier prediction that although the same serotype by VP7 reactivity, B641 and NCDV represent different VP4 serotypes. This difference in VP4 may have contributed to the lack of homotypic protection observed in calves, implicating VP4 as an important antigen in the active immune response to rotavirus infection in bovines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burns J. W., Chen D., Estes M. K., Ramig R. F. Biological and immunological characterization of a simian rotavirus SA11 variant with an altered genome segment 4. Virology. 1989 Apr;169(2):427–435. doi: 10.1016/0042-6822(89)90168-2. [DOI] [PubMed] [Google Scholar]
  2. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Res. 1983 Jul 25;11(14):4689–4701. doi: 10.1093/nar/11.14.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., Chanock R. M. In vitro transcription of two human rotaviruses. J Virol. 1982 Sep;43(3):1032–1037. doi: 10.1128/jvi.43.3.1032-1037.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glass R. I., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985 Mar;141(2):292–298. doi: 10.1016/0042-6822(85)90260-0. [DOI] [PubMed] [Google Scholar]
  6. Gorziglia M., Aguirre Y., Hoshino Y., Esparza J., Blumentals I., Askaa J., Thompson M., Glass R. I., Kapikian A. Z., Chanock R. M. VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. J Gen Virol. 1986 Nov;67(Pt 11):2445–2454. doi: 10.1099/0022-1317-67-11-2445. [DOI] [PubMed] [Google Scholar]
  7. Gorziglia M., Green K., Nishikawa K., Taniguchi K., Jones R., Kapikian A. Z., Chanock R. M. Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol. 1988 Aug;62(8):2978–2984. doi: 10.1128/jvi.62.8.2978-2984.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A. Z., Chanock R. M. Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7039–7043. doi: 10.1073/pnas.83.18.7039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gorziglia M., Larralde G., Kapikian A. Z., Chanock R. M. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7155–7159. doi: 10.1073/pnas.87.18.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gorziglia M., Larralde G., Ward R. L. Neutralization epitopes on rotavirus SA11 4fM outer capsid proteins. J Virol. 1990 Sep;64(9):4534–4539. doi: 10.1128/jvi.64.9.4534-4539.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987 Nov;161(1):153–159. doi: 10.1016/0042-6822(87)90181-4. [DOI] [PubMed] [Google Scholar]
  12. Kang S. Y., Saif L. J., Miller K. L. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol. 1989 Dec;27(12):2744–2750. doi: 10.1128/jcm.27.12.2744-2750.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kantharidis P., Dyall-Smith M. L., Holmes I. H. Marked sequence variation between segment 4 genes of human RV-5 and simian SA 11 rotaviruses. Arch Virol. 1987;93(1-2):111–121. doi: 10.1007/BF01313897. [DOI] [PubMed] [Google Scholar]
  14. Kantharidis P., Dyall-Smith M. L., Tregear G. W., Holmes I. H. Nucleotide sequence of UK bovine rotavirus segment 4: possible host restriction of VP3 genes. Virology. 1988 Oct;166(2):308–315. doi: 10.1016/0042-6822(88)90501-6. [DOI] [PubMed] [Google Scholar]
  15. López S., Arias C. F., Bell J. R., Strauss J. H., Espejo R. T. Primary structure of the cleavage site associated with trypsin enhancement of rotavirus SA11 infectivity. Virology. 1985 Jul 15;144(1):11–19. doi: 10.1016/0042-6822(85)90300-9. [DOI] [PubMed] [Google Scholar]
  16. López S., Arias C. F., Méndez E., Espejo R. T. Conservation in rotaviruses of the protein region containing the two sites associated with trypsin enhancement of infectivity. Virology. 1986 Oct 15;154(1):224–227. doi: 10.1016/0042-6822(86)90445-9. [DOI] [PubMed] [Google Scholar]
  17. López S., Arias C. F. The nucleotide sequence of the 5' and 3' ends of rotavirus SA11 gene 4. Nucleic Acids Res. 1987 Jun 11;15(11):4691–4691. doi: 10.1093/nar/15.11.4691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Dang M. N., Greenberg H. B. The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proc Natl Acad Sci U S A. 1988 Feb;85(3):645–649. doi: 10.1073/pnas.85.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matsuda Y., Nakagomi O., Offit P. A. Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains. Arch Virol. 1990;115(3-4):199–207. doi: 10.1007/BF01310530. [DOI] [PubMed] [Google Scholar]
  20. Matsui S. M., Mackow E. R., Greenberg H. B. Molecular determinant of rotavirus neutralization and protection. Adv Virus Res. 1989;36:181–214. doi: 10.1016/s0065-3527(08)60585-0. [DOI] [PubMed] [Google Scholar]
  21. Mitchell D. B., Both G. W. Complete nucleotide sequence of the simian rotavirus SA11 VP4 gene. Nucleic Acids Res. 1989 Mar 11;17(5):2122–2122. doi: 10.1093/nar/17.5.2122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nishikawa K., Taniguchi K., Torres A., Hoshino Y., Green K., Kapikian A. Z., Chanock R. M., Gorziglia M. Comparative analysis of the VP3 gene of divergent strains of the rotaviruses simian SA11 and bovine Nebraska calf diarrhea virus. J Virol. 1988 Nov;62(11):4022–4026. doi: 10.1128/jvi.62.11.4022-4026.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Potter A. A., Cox G., Parker M., Babiuk L. A. The complete nucleotide sequence of bovine rotavirus C486 gene 4 cDNA. Nucleic Acids Res. 1987 May 26;15(10):4361–4361. doi: 10.1093/nar/15.10.4361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 1986 Dec;155(2):434–451. doi: 10.1016/0042-6822(86)90205-9. [DOI] [PubMed] [Google Scholar]
  25. Snodgrass D. R., Fitzgerald T., Campbell I., Scott F. M., Browning G. F., Miller D. L., Herring A. J., Greenberg H. B. Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol. 1990 Mar;28(3):504–507. doi: 10.1128/jcm.28.3.504-507.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988 Jun;62(6):1870–1874. doi: 10.1128/jvi.62.6.1870-1874.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taniguchi K., Maloy W. L., Nishikawa K., Green K. Y., Hoshino Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. J Virol. 1988 Jul;62(7):2421–2426. doi: 10.1128/jvi.62.7.2421-2426.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woode G. N., Kelso N. E., Simpson T. F., Gaul S. K., Evans L. E., Babiuk L. Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves. J Clin Microbiol. 1983 Aug;18(2):358–364. doi: 10.1128/jcm.18.2.358-364.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Woode G. N., Zheng S. L., Rosen B. I., Knight N., Gourley N. E., Ramig R. F. Protection between different serotypes of bovine rotavirus in gnotobiotic calves: specificity of serum antibody and coproantibody responses. J Clin Microbiol. 1987 Jun;25(6):1052–1058. doi: 10.1128/jcm.25.6.1052-1058.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zheng S. L., Woode G. N., Melendy D. R., Ramig R. F. Comparative studies of the antigenic polypeptide species VP4, VP6, and VP7 of three strains of bovine rotavirus. J Clin Microbiol. 1989 Sep;27(9):1939–1945. doi: 10.1128/jcm.27.9.1939-1945.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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