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. 1986;64(1):113–119.

Comparative studies of Leishmania soluble antigens by crossed-immunoelectrophoresis: demonstration of specific antigens to L. mexicana and L. braziliensis*

Leonor L Leon, Michel van Hoegaerden, Bernardo Galvão-Castro
PMCID: PMC2490914  PMID: 2425997

Abstract

The antigenic relationship between two species of Leishmania (L. mexicana amazonensis and L. braziliensis braziliensis) was analysed using crossed-immunoelectrophoresis with an intermediate gel. Rabbit hyperimmune sera were prepared by subcutaneous inoculations of soluble antigens obtained from each species, emulsified in Freund's adjuvant. The antisera were then tested by immunodiffusion and electrophoresis; soluble antigens obtained from promastigote forms were submitted to ultrasonication and ultracentrifugation. Immunoelectrophoresis with homologous and heterologous antisera demonstrated the existence of some antigens which were species specific and some which were common to both species. The importance of these antigens is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chance M. L. The six diseases of WHO. Leishmaniasis. Br Med J (Clin Res Ed) 1981 Nov 7;283(6301):1245–1247. doi: 10.1136/bmj.283.6301.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clarke H. G., Freeman T. Quantitative immunoelectrophoresis of human serum proteins. Clin Sci. 1968 Oct;35(2):403–413. [PubMed] [Google Scholar]
  3. Handman E., Hocking R. E. Stage-specific, strain-specific, and cross-reactive antigens of Leishmania species identified by monoclonal antibodies. Infect Immun. 1982 Jul;37(1):28–33. doi: 10.1128/iai.37.1.28-33.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hunter K. W., Jr, Cook C. L. Leishmania mexicana: crossed immunoelectrophoretic analysis of culture-derived amastigotes and promastigotes. Trans R Soc Trop Med Hyg. 1983;77(2):177–180. doi: 10.1016/0035-9203(83)90060-3. [DOI] [PubMed] [Google Scholar]
  5. Kohanteb J., Ardehali S., Rezai H. R. Studies on antigenic relationships of Leishmania promastigotes by electroimmunodiffusion and crossed electroimmunodiffusion tests. Trans R Soc Trop Med Hyg. 1980;74(5):582–584. doi: 10.1016/0035-9203(80)90145-5. [DOI] [PubMed] [Google Scholar]
  6. McMahon-Pratt D., Bennett E., David J. R. Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis. J Immunol. 1982 Sep;129(3):926–927. [PubMed] [Google Scholar]
  7. Miles M. A., Póvoa M. M., de Souza A. A., Lainson R., Shaw J. J. Some methods for the enzymic characterization of Latin-American Leishmania with particular reference to Leishmania mexicana amazonensis and subspecies of Leishmania hertigi. Trans R Soc Trop Med Hyg. 1980;74(2):243–252. doi: 10.1016/0035-9203(80)90253-9. [DOI] [PubMed] [Google Scholar]
  8. Morgado M. G., van Hoegaerden M., Galvão-Castro B. Antígenos "particulares" a cepas do Trypanosoma cruzi: demonstraço por imunoeletroforese bidimensional. Mem Inst Oswaldo Cruz. 1982 Jan-Mar;77(1):59–67. doi: 10.1590/s0074-02761982000100007. [DOI] [PubMed] [Google Scholar]
  9. Schnur L. F., Zuckerman A., Greenblatt C. L. Leishmanial serotypes as distinguished by the gel diffusion of factors excreted in vitro and in vivo. Isr J Med Sci. 1972 Jul;8(7):932–942. [PubMed] [Google Scholar]
  10. Smrkovski L. L., Larson C. L. Antigenic cross-reactivity between Mycobacterium bovis (BCG) and Leishmania donovani. Infect Immun. 1977 Nov;18(2):561–562. doi: 10.1128/iai.18.2.561-562.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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