Skip to main content
NCBI home page
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1986;64(1):121–126.

Induction of delayed-type hypersensitivity in human volunteers immunized with a candidate leprosy vaccine consisting of killed Mycobacterium leprae

H K Gill, A S Mustafa, T Godal
PMCID: PMC2490923  PMID: 3524883

Abstract

A killed armadillo-derived Mycobacterium leprae vaccine was examined for its ability to induce a delayed-type hypersensitivity (DTH) response in purified protein derivative (PPD)-positive human volunteers living in a leprosy non-endemic country. Four groups of individuals aged between 23 and 28 years were given 1.5 × 107, 5 × 107, 1.5 × 108 and 5 × 108 M. leprae intradermally. A marked increase in reactivity to the M. leprae-derived skin test antigen (MLSA) was observed in the vaccinated groups receiving the three highest doses of vaccine while there was very little change observed in their PPD reactivity. No unacceptable side-effects attributable to the vaccine were observed. The killed armadillo-derived M. leprae vaccine thus appears to be able to induce a DTH response in man at doses which do not cause unacceptable side-effects.

Full text

PDF
121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Convit J., Aranzazu N., Ulrich M., Zúiga M., de Aragón M. E., Alvarado J., Reyes O. Investigations related to the development of a leprosy vaccine. Int J Lepr Other Mycobact Dis. 1983 Dec;51(4):531–539. [PubMed] [Google Scholar]
  2. Draper P. Cell walls of Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):95–98. [PubMed] [Google Scholar]
  3. Godal T. Immunological aspects of leprosy--present status. Prog Allergy. 1978;25:211–242. [PubMed] [Google Scholar]
  4. Harboe M., Closs O., Bjorvatn B., Kronvall G., Axelsen N. H. Antibody response in rabbits to immunization with Mycobacterium leprae. Infect Immun. 1977 Dec;18(3):792–805. doi: 10.1128/iai.18.3.792-805.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kirchheimer W. F., Storrs E. E. Attempts to establish the armadillo (Dasypus novemcinctus Linn.) as a model for the study of leprosy. I. Report of lepromatoid leprosy in an experimentally infected armadillo. Int J Lepr Other Mycobact Dis. 1971 Jul-Sep;39(3):693–702. [PubMed] [Google Scholar]
  6. Mehra V., Bloom B. R. Induction of cell-mediated immunity to Mycobacterium leprae in guinea pigs. Infect Immun. 1979 Mar;23(3):787–794. doi: 10.1128/iai.23.3.787-794.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mehra V., Brennan P. J., Rada E., Convit J., Bloom B. R. Lymphocyte suppression in leprosy induced by unique M. leprae glycolipid. Nature. 1984 Mar 8;308(5955):194–196. doi: 10.1038/308194a0. [DOI] [PubMed] [Google Scholar]
  8. Mitchison N. A. Immunology: rational design of vaccines. Nature. 1984 Mar 8;308(5955):113–114. doi: 10.1038/308112b0. [DOI] [PubMed] [Google Scholar]
  9. Mustafa A. S., Gill H. K., Nerland A., Britton W. J., Mehra V., Bloom B. R., Young R. A., Godal T. Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli. Nature. 1986 Jan 2;319(6048):63–66. doi: 10.1038/319063a0. [DOI] [PubMed] [Google Scholar]
  10. Myrvang B., Godal T., Ridley D. S., Fröland S. S., Song Y. K. Immune responsiveness to Mycobacterium leprae and other mycobacterial antigens throughout the clinical and histopathological spectrum of leprosy. Clin Exp Immunol. 1973 Aug;14(4):541–553. [PMC free article] [PubMed] [Google Scholar]
  11. Patel P. J., Lefford M. J. Induction of cell-mediated immunity to Mycobacterium leprae in mice. Infect Immun. 1978 Jan;19(1):87–93. doi: 10.1128/iai.19.1.87-93.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
  13. Shepard C. C., Draper P., Rees R. J., Lowe C. Effect of purification steps on the immunogenicity of Mycobacterium leprae. Br J Exp Pathol. 1980 Aug;61(4):376–379. [PMC free article] [PubMed] [Google Scholar]
  14. Shepard C. C. Vaccination of mice against M. leprae infection. Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):222–226. [PubMed] [Google Scholar]
  15. Shepard C. C., Walker L. L., van Landingham R. Heat stability of Mycobacterium leprae immunogenicity. Infect Immun. 1978 Oct;22(1):87–93. doi: 10.1128/iai.22.1.87-93.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shepard C. C., van Landingham R., Walker L. L. Searches among mycobacterial cultures for antileprosy vaccines. Infect Immun. 1980 Sep;29(3):1034–1039. doi: 10.1128/iai.29.3.1034-1039.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smelt A. H., Rees R. J., Liew F. Y. Induction of delayed-type hypersensitivity to Mycobacterium leprae in healthy individuals. Clin Exp Immunol. 1981 Jun;44(3):501–506. [PMC free article] [PubMed] [Google Scholar]
  18. Young D. B., Buchanan T. M. A serological test for leprosy with a glycolipid specific for Mycobacterium leprae. Science. 1983 Sep 9;221(4615):1057–1059. doi: 10.1126/science.6348948. [DOI] [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES