Abstract
An international collaborative study was carried out to identify monoclonal antibodies that could reliably discriminate between wild polioviruses and strains derived from Sabin vaccine viruses. For poliovirus types 2 and 3, monoclonal antibodies were identified that reacted specifically with type 2 or type 3 strains which gave T1-oligonucleotide maps similar to or indistinguishable from that of Sabin vaccine virus, thus indicating their vaccine origin. These monoclonal antibodies failed to react with strains which gave T1 maps unrelated to that of Sabin vaccine virus. However for type 1, five of the six antibodies examined in the study reacted only with strains with a T1 map indistinguishable from that of type 1 Sabin vaccine virus. In contrast, other monoclonal antibodies against poliovirus types 1, 2 and 3 reacted broadly within a serotype.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crainic R., Couillin P., Blondel B., Cabau N., Boué A., Horodniceanu F. Natural variation of poliovirus neutralization epitopes. Infect Immun. 1983 Sep;41(3):1217–1225. doi: 10.1128/iai.41.3.1217-1225.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferguson M., Yi-hua Q., Minor P. D., Magrath D. I., Spitz M., Schild G. C. Monoclonal antibodies specific for the Sabin vaccine strain of poliovirus 3. Lancet. 1982 Jul 17;2(8290):122–124. doi: 10.1016/s0140-6736(82)91091-1. [DOI] [PubMed] [Google Scholar]
- Kew O. M., Nottay B. K., Hatch M. H., Nakano J. H., Obijeski J. F. Multiple genetic changes can occur in the oral poliovaccines upon replication in humans. J Gen Virol. 1981 Oct;56(Pt 2):337–347. doi: 10.1099/0022-1317-56-2-337. [DOI] [PubMed] [Google Scholar]
- Minor P. D. Characterization of strains of type 3 poliovirus by oligonucleotide mapping. J Gen Virol. 1982 Apr;59(Pt 2):307–317. doi: 10.1099/0022-1317-59-2-307. [DOI] [PubMed] [Google Scholar]
- Osterhaus A. D., van Wezel A. L., van Steenis B., Drost G. A., Hazendonk T. G. Monoclonal antibodies to polioviruses. Production of specific monoclonal antibodies to the Sabin vaccine strains. Intervirology. 1981;16(4):218–224. doi: 10.1159/000149270. [DOI] [PubMed] [Google Scholar]