Abstract
Use of the single-radial-haemolysis (SRH) technique for the diagnosis of flavivirus infections is described. A large number of paired and single convalescent serum samples collected from cases of encephalitis during two major outbreaks in Kolar district of Karnataka State in India during 1977 and 1979 were tested by this technique. The results were compared with those obtained in the haemagglutination inhibition (HI) test in all cases, and the complement fixation (CF) and neutralization tests in some cases. Japanese encephalitis virus was shown by the SRH test to be the major etiologic agent responsible for both epidemics. This was corroborated by the HI, CF and neutralization test results. The single-radial-haemolysis test was found to be simpler and more specific and sensitive than the haemagglutination inhibition test.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Duca M., Duca E., Ionescu L., Abdalla H. Single haemolysis for the assay of antibodies to some haemagglutinating arboviruses. Bull World Health Organ. 1979;57(6):937–942. [PMC free article] [PubMed] [Google Scholar]
- Gaidamovich S. Y., Melnikova E. E. Passive hemolysis-in-gel with Togaviridae arboviruses. Intervirology. 1980;13(1):16–20. doi: 10.1159/000149101. [DOI] [PubMed] [Google Scholar]
- Gaidamovich S. Y., Siddiqi S. M., Haq F., Klisenko G. A., Melnikova E. E., Obukhova V. R. Serological evidence of dengue fever in the Bangladesh Republic. Acta Virol. 1980 Mar;24(2):153–153. [PubMed] [Google Scholar]
- Odelola H. A. Application of single-radial-haemolysis test for the detection of antibodies to togaviruses. Arch Virol. 1979;60(3-4):325–328. doi: 10.1007/BF01317503. [DOI] [PubMed] [Google Scholar]
- PAVRI K. M., GOKHALE T., SHAH K. V. Serological response to Russian spring-summer encephalitis virus vaccine as measured with Kyasanur Forest disease virus. Indian J Med Res. 1962 Mar;50:153–161. [PubMed] [Google Scholar]