Abstract
Enzyme-linked immunosorbent asays (ELISAs) are described for determining levels of dapsone and pyrimethamine in urine. Both assays have a sensitivity of about 20 μg/l and are reproducible, but each produces some false positives. The problem of false positive reactions was partially obviated by requiring positive results in both assays. In a pilot study involving 50 children aged 3 months to 4 years who were given a single dose of Maloprim (pyrimethamine + dapsone), 75% were positive for dapsone 7 days after administration of the drug, while 25% were still positive 15 days after its administration. The corresponding proportions for pyrimethamine were 73% and 30%, respectively. Comparison of the results obtained in a larger chemoprophylaxis trial with those from the pilot study indicated that the assays described could be used to investigate whether antimalarials had been taken.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahmad R. A., Rogers H. J. Pharmacokinetics and protein binding interactions of dapsone and pyrimethamine. Br J Clin Pharmacol. 1980 Nov;10(5):519–524. doi: 10.1111/j.1365-2125.1980.tb01798.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huikeshoven H., de Wit M., Soeters A., Landheer J., Leiker D. L. ELISA inhibition technique for the demonstration of sulphones in body fluids. II. A new method to monitor leprosy patient compliance under field conditions. Lepr Rev. 1981 Mar;52(1):11–18. [PubMed] [Google Scholar]
- Jones C. R., Ovenell S. M. Determination of plasma concentrations of dapsone, monoacetyl dapsone and pyrimethamine in human subjects dosed with maloprim. J Chromatogr. 1979 Jun 11;163(2):179–185. doi: 10.1016/s0378-4347(00)81461-9. [DOI] [PubMed] [Google Scholar]
- Lelijveld J., Kortmann H. The eosin colour test of Dill and Glazko: a simple field test to detect chloroquine in urine. Bull World Health Organ. 1970;42(3):477–479. [PMC free article] [PubMed] [Google Scholar]
- Verdier F., Ramanamirija J. A., Pussard E., Clavier F., Biaud J. M., Coulanges P., Le Bras J. Unreliability of Dill Glazko test in detecting chloroquine in urine. Lancet. 1985 Jun 1;1(8440):1282–1283. doi: 10.1016/s0140-6736(85)92358-x. [DOI] [PubMed] [Google Scholar]