Abstract
The safety and immunogenicity of two live influenza A virus vaccine strains, the CR 59 and 17/25/1 cold-adapted (ca) reassortants, were evaluated in 170 healthy young adult volunteers. The vaccines were produced by recombining A/Korea/1/82 (H3N2) wild-type virus with either A/Ann Arbor/6/60 (H2N2) or A/Leningrad/134/17/57 (H2N2) ca donors of attenuation. Both vaccines were well tolerated in volunteers. The 17/25/1 strain, prepared from A/Leningrad, infected at least 70% of seronegative volunteers after the first dose and 84% after the second; the CR 59 strain infected 62% and 72% of volunteers after first and second doses, respectively. Among the vaccinees who were initially seropositive, 17/25/1 infected 66% after one dose and 85% after two, while CR 59 infected 62% and 71%, respectively. Despite differences in temperature sensitivity, genetic composition, and serological reactivity to monoclonal antibodies, both vaccines behaved almost identically in animal models and man. We conclude that both donors of attenuation may be of great potential value.
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Selected References
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- Betts R. F., Douglas R. G., Jr, Murphy B. R. Resistance to challenge with influenza A/Hong Kong/123/77 (H1N1) wild-type virus induced by live attenuated A/Hong Kong/123/77 (H1N1) cold-adapted reassortant virus. J Infect Dis. 1985 Apr;151(4):744–745. doi: 10.1093/infdis/151.4.744. [DOI] [PubMed] [Google Scholar]
- Clements M. L., Betts R. F., Murphy B. R. Advantage of live attenuated cold-adapted influenza A virus over inactivated vaccine for A/Washington/80 (H3N2) wild-type virus infection. Lancet. 1984 Mar 31;1(8379):705–708. doi: 10.1016/s0140-6736(84)92222-0. [DOI] [PubMed] [Google Scholar]
- Cox N. J., Maassab H. F., Kendal A. P. Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25 degrees between recent H3N2 and H1N1 epidemic strains and cold-adapted A/An Arbor/6/60. Virology. 1979 Aug;97(1):190–194. doi: 10.1016/0042-6822(79)90386-6. [DOI] [PubMed] [Google Scholar]
- Hoskins T. W., Davies J. R., Smith A. J., Miller C. L., Allchin A. Assessment of inactivated influenza-A vaccine after three outbreaks of influenza A at Christ's Hospital. Lancet. 1979 Jan 6;1(8106):33–35. doi: 10.1016/s0140-6736(79)90468-9. [DOI] [PubMed] [Google Scholar]
- Jennings R., Potter C. W., Teh C. Z., Mahmud M. I. The replication of type A influenza viruses in the infant rat: a marker for virus attenuation. J Gen Virol. 1980 Aug;49(2):343–354. doi: 10.1099/0022-1317-49-2-343. [DOI] [PubMed] [Google Scholar]
- Luther P., Bergmann K. C., Oxford J. S. An investigation of antigenic drift of neuraminidases of influenza A (H1N1) viruses. J Hyg (Lond) 1984 Apr;92(2):223–229. doi: 10.1017/s002217240006424x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maassab H. F. Adaptation and growth characteristics of influenza virus at 25 degrees c. Nature. 1967 Feb 11;213(5076):612–614. doi: 10.1038/213612a0. [DOI] [PubMed] [Google Scholar]
- Oxford J. S., Corcoran T., Newman R., Major D., Schild G. C. Biochemical and antigenic analysis using monoclonal antibodies of a series of of influenza A (H3N2) and (H1N1) virus reassortants. Vaccine. 1986 Mar;4(1):9–14. doi: 10.1016/0264-410x(86)90091-5. [DOI] [PubMed] [Google Scholar]
- Potter C. W., Oxford J. S., Shore S. L., McLaren C., Stuart-Harris C. Immunity to influenza in ferrets. I. Response to live and killed virus. Br J Exp Pathol. 1972 Apr;53(2):153–167. [PMC free article] [PubMed] [Google Scholar]