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. 1989;67(3):295–300.

Adaptations of the Saker-Solomons test: simple, reliable colorimetric field assays for chloroquine and its metabolites in urine.

D L Mount, B L Nahlen, L C Patchen, F C Churchill
PMCID: PMC2491246  PMID: 2766451

Abstract

Two field-adapted colorimetric methods for measuring the antimalarial drug chloroquine in urine are described. Both are modifications of the method of Saker and Solomons for screening urine for phencyclidine and other drugs of abuse, using the colour reagent tetrabromophenolphthalein ethyl ester. One method is semiquantitative, detecting the presence of chloroquine (Cq) and its metabolites in urine with a 1 microgram/ml detection limit; it is more sensitive and reliable than the commonly used Dill-Glazko method and is as easy to apply in the field. The second method uses a hand-held, battery-operated filter photometer to quantify Cq and its metabolites with a 2 microgram/ml detection limit and a linear range up to 8 micrograms/ml. The first method was validated in the field using a published quantitative colorimetric method and samples from a malaria study in Nigeria. The second method was validated in the laboratory against high-performance liquid chromatographic results on paired samples from the Nigerian study. Both methods may be used in remote locations where malaria is endemic and no electricity is available.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergqvist Y., Hed C., Funding L., Suther A. Determination of chloroquine and its metabolites in urine: a field method based on ion-pair extraction. Bull World Health Organ. 1985;63(5):893–898. [PMC free article] [PubMed] [Google Scholar]
  2. Lelijveld J., Kortmann H. The eosin colour test of Dill and Glazko: a simple field test to detect chloroquine in urine. Bull World Health Organ. 1970;42(3):477–479. [PMC free article] [PubMed] [Google Scholar]
  3. Mount D. L., Nahlen B. L., Patchen L. C., Churchill F. C. Field-adapted method for high-performance thin-layer chromatographic detection and estimation of chloroquine and desethylchloroquine in urine. J Chromatogr. 1987 Dec 25;423:261–269. doi: 10.1016/0378-4347(87)80349-3. [DOI] [PubMed] [Google Scholar]
  4. Mount D. L., Patchen L. C., Churchill F. C. Field-adapted method for high-performance thin-layer chromatographic detection and estimation of chloroquine in finger-stick blood. J Chromatogr. 1988 Jun 24;428(1):196–202. doi: 10.1016/s0378-4347(00)83908-0. [DOI] [PubMed] [Google Scholar]
  5. Mount D. L., Patchen L. C., Williams S. B., Churchill F. C. Colorimetric and thin-layer chromatographic methods for field assay of chloroquine and its metabolites in urine. Bull World Health Organ. 1987;65(5):615–623. [PMC free article] [PubMed] [Google Scholar]
  6. Patchen L. C., Mount D. L., Schwartz I. K., Churchill F. C. Analysis of filter-paper-absorbed, finger-stick blood samples for chloroquine and its major metabolite using high-performance liquid chromatography with fluorescence detection. J Chromatogr. 1983 Nov 11;278(1):81–89. doi: 10.1016/s0378-4347(00)84758-1. [DOI] [PubMed] [Google Scholar]
  7. Rio J. G., Hodnett C. N. Evaluation of a colorimetric screening test for basic drugs in urine. J Anal Toxicol. 1981 Nov-Dec;5(6):267–269. doi: 10.1093/jat/5.6.267. [DOI] [PubMed] [Google Scholar]
  8. Rombo L., Björkman A., Sego E., Lindström B., Ericsson O., Gustafsson L. L. Evaluation of three qualitative tests for detection of chloroquine in urine--agreement with plasma concentrations determined with liquid chromatography. Ann Trop Med Parasitol. 1986 Jun;80(3):293–298. doi: 10.1080/00034983.1986.11812019. [DOI] [PubMed] [Google Scholar]
  9. Rombo L., Björkman A., Sego E., Lindström B., Ericsson O., Gustafsson L. L. Reliability of Dill-Glazko test. Lancet. 1985 Jun 29;1(8444):1509–1509. doi: 10.1016/s0140-6736(85)92284-6. [DOI] [PubMed] [Google Scholar]
  10. Steketee R. W., Mount D. L., Patchen L. C., Williams S. B., Churchill F. C., Roberts J. M., Kaseje D. C., Brandling-Bennett A. D. Field application of a colorimetric method of assaying chloroquine and desethylchloroquine in urine. Bull World Health Organ. 1988;66(4):485–490. [PMC free article] [PubMed] [Google Scholar]
  11. WILSON T., EDESON J. F. Studies on the chemotherapy of malaria. III. The treatment of acute malaria with chloroquine. Med J Malaya. 1954 Dec;9(2):115–131. [PubMed] [Google Scholar]

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