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. 1990 Feb;64(2):700–705. doi: 10.1128/jvi.64.2.700-705.1990

Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus.

F Kobune 1, H Sakata 1, A Sugiura 1
PMCID: PMC249163  PMID: 2153236

Abstract

B95-8, an Epstein-Barr virus-transformed marmoset B-lymphoblastoid cell line, and its derivative B95a, capable of attachment to a substrate surface, were 10,000-fold more sensitive to measles virus present in clinical specimens than were Vero cells. B95-8 and B95a cells were thus thought to be useful host cells for the isolation of measles virus. Quantitation of measles virus present in clinical specimens showed that a large quantity of virus, exceeding 10(6) 50% tissue culture infective doses per ml of a nasal-swab eluate, is shed into secretions by patients with acute measles, consistent with the contagiousness of the disease. Measles viruses isolated in B95a cells differed in some biological properties from those adapted to Vero cells. First, the viruses isolated in B95a cells did replicate in Vero cells, but release into the fluid phase was less efficient than that of Vero cell-adapted viruses. Second, minor antigenic differences were found between virus strains isolated in B95a cells and those isolated in Vero cells from the same clinical specimens. Third, the viruses isolated and propagated in B95a cells caused clinical signs in experimentally infected monkeys resembling those of human measles. It was suspected that measles virus is subject to host cell-mediated selection and that the viruses grown in B95a cells are more representative of measles virus circulating among humans than are the viruses selected in Vero cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrecht P., Lorenz D., Klutch M. J., Vickers J. H., Ennis F. A. Fatal measles infection in marmosets pathogenesis and prophylaxis. Infect Immun. 1980 Mar;27(3):969–978. doi: 10.1128/iai.27.3.969-978.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ENDERS J. F., PEEBLES T. C. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc Soc Exp Biol Med. 1954 Jun;86(2):277–286. doi: 10.3181/00379727-86-21073. [DOI] [PubMed] [Google Scholar]
  3. Hall W. C., Kovatch R. M., Herman P. H., Fox J. G. Pathology of measles in rhesus monkeys. Vet Pathol. 1971;8(4):307–319. doi: 10.1177/030098587100800403. [DOI] [PubMed] [Google Scholar]
  4. Katz J. M., Naeve C. W., Webster R. G. Host cell-mediated variation in H3N2 influenza viruses. Virology. 1987 Feb;156(2):386–395. doi: 10.1016/0042-6822(87)90418-1. [DOI] [PubMed] [Google Scholar]
  5. Katz J. M., Webster R. G. Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual. Virology. 1988 Aug;165(2):446–456. doi: 10.1016/0042-6822(88)90588-0. [DOI] [PubMed] [Google Scholar]
  6. Levy B. M., Mirkovic R. R. An epizootic of measles in a marmoset colony. Lab Anim Sci. 1971 Feb;21(1):33–39. [PubMed] [Google Scholar]
  7. McChesney M. B., Fujinami R. S., Lerche N. W., Marx P. A., Oldstone M. B. Virus-induced immunosuppression: infection of peripheral blood mononuclear cells and suppression of immunoglobulin synthesis during natural measles virus infection of rhesus monkeys. J Infect Dis. 1989 Apr;159(4):757–760. doi: 10.1093/infdis/159.4.757. [DOI] [PubMed] [Google Scholar]
  8. Miller G., Shope T., Lisco H., Stitt D., Lipman M. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc Natl Acad Sci U S A. 1972 Feb;69(2):383–387. doi: 10.1073/pnas.69.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Monroe J. E., Calender A., Mulder C. Epstein-Barr virus-positive and -negative B-cell lines can be infected with human immunodeficiency virus types 1 and 2. J Virol. 1988 Sep;62(9):3497–3500. doi: 10.1128/jvi.62.9.3497-3500.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Potkay S., Ganaway J. R., Rogers N. G., Kinard R. An epizootic of measles in a colony of rhesus monkeys (Macaca mulatta). Am J Vet Res. 1966 Jan;27(116):331–334. [PubMed] [Google Scholar]
  11. Pyhälä R., Pyhälä L., Pekkala P. Host cell-mediated selection of influenza A (H3N2) virus variant subpopulations: lack of association between antigenic and receptor-binding properties. Epidemiol Infect. 1988 Jun;100(3):511–522. doi: 10.1017/s0950268800067248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robertson J. S., Bootman J. S., Newman R., Oxford J. S., Daniels R. S., Webster R. G., Schild G. C. Structural changes in the haemagglutinin which accompany egg adaptation of an influenza A(H1N1) virus. Virology. 1987 Sep;160(1):31–37. doi: 10.1016/0042-6822(87)90040-7. [DOI] [PubMed] [Google Scholar]
  13. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs. Virology. 1985 May;143(1):166–174. doi: 10.1016/0042-6822(85)90105-9. [DOI] [PubMed] [Google Scholar]
  14. Sato T. A., Fukuda A., Sugiura A. Characterization of major structural proteins of measles virus with monoclonal antibodies. J Gen Virol. 1985 Jul;66(Pt 7):1397–1409. doi: 10.1099/0022-1317-66-7-1397. [DOI] [PubMed] [Google Scholar]
  15. Sato T. A., Kohama T., Sugiura A. Intracellular processing of measles virus fusion protein. Arch Virol. 1988;98(1-2):39–50. doi: 10.1007/BF01321004. [DOI] [PubMed] [Google Scholar]
  16. Schild G. C., Oxford J. S., de Jong J. C., Webster R. G. Evidence for host-cell selection of influenza virus antigenic variants. Nature. 1983 Jun 23;303(5919):706–709. doi: 10.1038/303706a0. [DOI] [PubMed] [Google Scholar]
  17. Yamanouchi K., Chino F., Kobune F., Kodama H., Tsuruhara T. Growth of measles virus in the lymphoid tissues of monkeys. J Infect Dis. 1973 Dec;128(6):795–799. doi: 10.1093/infdis/128.6.795. [DOI] [PubMed] [Google Scholar]

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