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. 1990 Feb;64(2):950–956. doi: 10.1128/jvi.64.2.950-956.1990

Phenotypic analysis of bovine papillomavirus type 1 E2 repressor mutants.

P F Lambert 1, B C Monk 1, P M Howley 1
PMCID: PMC249197  PMID: 2153256

Abstract

The bovine papillomavirus type 1 (BPV-1) E2 open reading frame encodes three proteins: the E2 transcriptional transactivator, the E2 transcriptional repressor (E2-TR), and the E8/E2 fusion peptide. In this study, we describe the phenotypes of BPV-1 mutants which are disrupted in their capacity to encode either the E2 transcriptional repressor or the E8/E2 fusion peptide. We also describe experiments which demonstrate that the E8/E2 gene product functions similarly to E2-TR. In the context of the entire viral genome, disruption of E8/E2 expression had little effect on viral processes, whereas disruption of E2-TR expression resulted in a complex phenotype marked by a 10- to 20-fold increase in viral DNA plasmid copy number as well as increased transformation potential. A double mutant, defective in the expression of both E8/E2 and E2-TR proteins, had high levels of E2 transactivation activity yet had reduced plasmid replication capacity and a delayed capacity to transform rodent cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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