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. 1990 Mar;64(3):1201–1206. doi: 10.1128/jvi.64.3.1201-1206.1990

An antigen chimera of poliovirus induces antibodies against human papillomavirus type 16.

O Jenkins 1, J Cason 1, K L Burke 1, D Lunney 1, A Gillen 1, D Patel 1, D J McCance 1, J W Almond 1
PMCID: PMC249234  PMID: 2154604

Abstract

It has been established that the surface of poliovirus type 1 can be extensively modified to incorporate antigenic domains from other poliovirus serotypes and from unrelated viruses. The fact that the modified (chimeric) viruses exhibit dual antigenicity and immunogenicity led us to explore the possibility of using the Sabin vaccine strain of poliovirus type 1 as a vector for the presentation of antigenic domains from human papillomavirus type 16 (HPV-16), a virus associated with the development of cervical carcinoma. We report here the construction and characterization of a chimeric poliovirus containing a 16-residue sequence derived from the major capsid protein (L1) of HPV-16. This virus chimera stimulated the production in rabbits of antibodies which recognized the HPV-16-derived peptide and an L1 fusion protein synthesized in Escherichia coli and detected HPV-16 in human biopsy material by immunoperoxidase staining. The possibility that poliovirus-HPV chimeras could be used as vaccines against HPV-16 is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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